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| Status |
Public on Aug 22, 2016 |
| Title |
Gene expression profiling of mouse spleen XCR1+ DC at steady state, 3 hours after polyI:C or 12 hours after STAg i.v. injection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The goal of this experiment was to use global gene expression profiling to compare the global genetic reprogramming of spleen XCR1+ DC upon in vivo stimulation with a viral-type ligand, polyI:C which strongly induces type I interferons, versus with a ligand derived from an intracellular parasite which strongly induces IFN-g.
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| Overall design |
DC were isolated as previously described (Robbins SH et al. Genome Biol. 2008. PMID: 18218067; Baranek T et al. Cell Host Microbe. 2012. PMID: 23084923), from the spleens of wild-type C57BL/6 mice 12 hours after i.v. injection of 20µg of STAg. DC subsets were sorted by flow cytometry according to the marker combinations described in the “characteristics: phenotype” field for each sample. The data for spleen XCR1+ DC isolated from untreated control animals or from mice that had been injected i.v. with 100µg polyI:C 3 hours before organ harvest have been previously published and are available in the GEO database GSE39556 series. The complete dataset representing: (1) the Samples corresponding to STAg stimulation and (2) the untreated control Samples and the Samples corresponding to polyI:C stimulation from Series GSE39556 (re-processed using RMA), is linked below as a supplementary file.
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| Contributor(s) |
Alexandre Y, Chelbi R, Carpentier S, Crozat K, Dalod M |
| Citation(s) |
27533013 |
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| Submission date |
Jul 21, 2015 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Marc DALOD |
| E-mail(s) |
dalod@ciml.univ-mrs.fr
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| Organization name |
CIML
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| Street address |
Parc Scientifique et Technologique de Luminy, 163 avenue de Luminy, Case 906, 13288 Marseille cedex 9
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| City |
MARSEILLE |
| ZIP/Postal code |
13009 |
| Country |
France |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE71171 |
Broad and largely overlapping molecular changes arise in thymic and peripheral XCR1+ dendritic cells upon tolerogenic and immunogenic maturation |
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| Relations |
| BioProject |
PRJNA290491 |
