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Series GSE71162 Query DataSets for GSE71162
Status Public on Oct 05, 2015
Title A mechanism for expansion of the regulatory T cell repertoire and its role in enforcing self-tolerance
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To investigate the influence of CNS3, a cis-regulatory element in the Foxp3 locus, on the selection of T cell antigen receptor (TCR) repertoire of regulator CD4+ T cells (Treg), we crossed Foxp3ΔCNS3-gfp or control Foxp3gfp mice to DO11.10 TCRβ transgene and Tcra-/+ background. We isolated Treg and conventional CD4+T cells from thymus, spleen and lymph nodes of Foxp3ΔCNS3-gfp DO11.10 TCRβ Tcra-/+ or Foxp3gfp DO11.10 TCRβ Tcra-/+ male littermates, and sequenced the TCRα chains. Analysis of the diversity of Complementary Determining Region 3 (CDR3) of TCRα showed a distinct clustering of CNS3-deficient Treg cells from the CNS3-sufficient ones.
Overall design DO11.10 TCRβ transgene inhibits the recombination of endogenous Tcrb loci thus restricting TCR repertoire to TCRα chains expressed by T cells. Further limitation of the TCR repertoire was achieved by the presence of one functional Tcra gene. With restricted TCR repertoire, mRNA of TCRα was extracted from Treg and conventional CD4+ T cells for library preparation and high throughput sequencing.
Contributor(s) Feng Y, Rudensky AY
Citation(s) 26605529
Submission date Jul 21, 2015
Last update date May 15, 2019
Contact name Alexander Y Rudensky
Organization name Memorial Sloan Kettering Cancer Center
Department Immunology
Street address 408 E 69th ST Z-1445
City New York
State/province NY
ZIP/Postal code 10021
Country USA
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (63)
GSM1828673 RX142_LN_Te
GSM1828674 RX161_LN_Te
GSM1828675 RX164_LN_Te
BioProject PRJNA290476
SRA SRP061377

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