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| Status |
Public on May 12, 2016 |
| Title |
Injury-induced enhancer remodelling in spinal microglia - a novel mechanism for pain chronification? [RNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Chronic pain is common and devastating. Yet, its precise molecular origins remain unclear. The condition induces well-characterised changes in neurons and microglia, but it is unknown why they persist long after the precipitating injury has healed. We posit a role for enhancers - regions of open chromatin that define a cell’s transcription factor binding profile. Enhancer profiles can alter upon environmental stimulation, functioning as a kind of molecular memory. Here, a mouse model of persistent neuropathic pain was used to examine microglial enhancers with flow cytometry and sequencing. We observed injury-specific alterations of enhancers in close proximity to transcriptionally regulated genes. Our data also shine light on details relating to the spinal cord immune response and provide the first genome-wide gene expression profile of isolated microglia in a pain state. We hypothesise that enhancer deposition may constitute a novel mechanism by which painful experiences are encoded on a molecular level.
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| |
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| Overall design |
ChIP-seq and RNA-seq of isolated spinal cord microglia after peripheral spinal nerve ligation or sham surgery in mice (day 7)
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| |
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| Contributor(s) |
Denk F, Crow M, Didangelos A, Lopes DM, McMahon SB |
| Citation(s) |
27184839 |
| |
| Submission date |
Jul 21, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Franziska Denk |
| Organization name |
King's College London
|
| Street address |
Guy's Campus
|
| City |
London |
| ZIP/Postal code |
SE1 1UL |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE71136 |
Injury-induced enhancer remodelling in spinal microglia - a novel mechanism for pain chronification? |
|
| Relations |
| BioProject |
PRJNA290437 |
| SRA |
SRP061355 |
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