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Series GSE70981 Query DataSets for GSE70981
Status Public on Jan 18, 2016
Title Blimp1 controls plasma cell physiology and function
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Antibody-secreting plasma cells are the terminal stage of the B-cell lineage. Plasma cell differentiation requires a major resetting of gene expression to silence the B cell transcriptional program, whilst establishing secretory function and long-term survival. The transcription factors Blimp1 and Irf4 are essential for the initial differentiation of activated B cells to antibody-secreting cells, however their function in mature plasma cells remains elusive. We have found that while Irf4 was essential for plasma cell survival, Blimp1 was dispensable. Blimp1-deficient cells retained the unique plasma cell transcriptional signature, but lost the ability to secrete antibody or to maintain the characteristic size and ultrastructure of plasma cells. Blimp1 was required for full expression of many components of the unfolded protein response (UPR), including Xbp1 and Atf6, as well as for the appropriate processing of Igh mRNA. The overlap of Blimp1 and Xbp1 function was restricted to the UPR genes, with Blimp1 uniquely regulating activity of the mTOR pathway, plasma cell size and morphology. These studies establish Blimp1 as a major regulator of the UPR pathway that is also required for the unique metabolic requirements of plasma cells enabling the secretion of protective antibody.
Overall design RNA-seq was performed on wild type, Blimp1-/- and Xbp1-/- mouse plasma cells. Between two to four biological replicates were generated and sequenced for each sample.
Contributor(s) Shi W, Tellier J, Liao Y, Smyth GK, Nutt SL
Citation(s) 26779600
Submission date Jul 15, 2015
Last update date May 15, 2019
Contact name Wei Shi
Organization name The Walter and Eliza Hall Institute of Medical Research
Department Bioinformatics
Street address 1G Royal Parade
City Parkville
State/province Victoria
ZIP/Postal code 3050
Country Australia
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (8)
GSM1824689 Wild type (WT) rep1, batch 1
GSM1824690 Wild type (WT) rep2, batch 1
GSM1824691 Wild type (WT) rep3, batch 2
SRA SRP061216
BioProject PRJNA290087

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE70981_GEO_suppl_raw_counts.txt.gz 400.9 Kb (ftp)(http) TXT
GSE70981_RAW.tar 1.7 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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