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| Status |
Public on Jul 15, 2015 |
| Title |
siRNA screen for modifiers of Smad4 nuclear translocation |
| Organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
The TGFb signaling pathway is a critical regulator of developmental processes and disease. We developed a system to measure TGFb signaling dynamics by monitoring the localization of Smad4.
We performed an unbiased genome-wide RNAi screen to identify genes involved in ligand-dependent Smad4 nuclear translocation
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| Overall design |
C2C12 GFP-Smad4 reporter cells were transfected with pools of 3 siRNAs targeting individual genes; after 48 hours, cells were stimulated with 2ng/ml TGFb1 for 1 or 6 hours, then fixed and analyzed for Smad4 localization. Two independent experiments were performed, screening 17,582 genes. On each plate, two control wells (siRNA targeting TGFbR1 and XpoI) were included
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| Contributor(s) |
Deglincerti A, Haremaki T, Warmflash A, Sorre B, Brivanlou AH |
| Citation(s) |
26116664 |
| |
| Submission date |
Jul 14, 2015 |
| Last update date |
Sep 04, 2015 |
| Contact name |
Alessia Deglincerti |
| Organization name |
The Rockefeller University
|
| Street address |
1230 York Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA289930 |
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