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| Status |
Public on Jul 09, 2015 |
| Title |
Expression data from foam cells of apolipoprotein E-deficient mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Hypercholesterolemai is a major contributor to atherosclerosis development. To assess the effects of hypercholesterolemia on the transcriptional profiling in foam cells, mice were fed regular chow, or WD for 2 or 14 weeks prior to sacrifice.
We used microarrays for broad analysis of gene expression in the 3 experimental groups. Each group included 5 mice.
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| Overall design |
ApoE-/- mice were fed regular mouse chow to the age of 22 weeks (CHOW) or a western-type diet for 2 weeks between weeks 22 and 24 (2w-WD) or for 14 weeks between weeks 8 and 22 (14w-WD). Foam cells in cross-sections of the aourtic sinus were isolated by laser-capsure microdissection (n=5 per group). RNA was amplified by in vitro transcription, biotinylated, and hybridized to Affymetrix Mouse Genome 430 2.0 Arrays.
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| Contributor(s) |
Paul A |
| Citation(s) |
27091181, 27408807 |
| |
| Submission date |
Jul 08, 2015 |
| Last update date |
Oct 03, 2019 |
| Contact name |
Antonio Paul |
| E-mail(s) |
PAULA@MAIL.AMC.EDU
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| Phone |
518-262-1158
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| Organization name |
Albany Medical College
|
| Street address |
Center for Cardiovascular Sciences
|
| City |
Albany |
| State/province |
NY |
| ZIP/Postal code |
12208 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA289194 |
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