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Series GSE70605 Query DataSets for GSE70605
Status Public on May 17, 2016
Title Single Cell Sequencing Identifies Key Epigenetic Regulators in Nuclear Transfer Mediated Reprogramming [RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Differentiated cell can be reprogrammed into totipotent embryo through somatic cell nuclear transfer (SCNT). However, this process is highly inefficient and most cloned embryos arrest at certain developmental stages. Through single cell sequencing combined with embryo biopsy, here we generate a global map of DNA methylome and RNA transcriptome for SCNT embryos with distinct developmental fates. We subsequently demonstrate that the unfaithful reactivation of two histone demethylases, Kdm4b and Kdm5b, accounts for the arrest of cloned embryos at 2-cell and 4-cell stage, respectively. Ectopic expression of Kdm4b and Kdm5b in SCNT can remove H3K9me3 barrier, restore the transcription profile and facilitate the blastocyst developmental efficiency over 95%. Moreover, these cloned embryos can further support full-term development and the derivation of SCNT-embryonic stem cells with greater efficiency. Our study reveals that histone methylation reset is crucial for the development of SCNT embryos, which provides a clue to further improve therapeutic cloning.
 
Overall design For SCNT embryos or injected SCNT embryos 3-8 replicates were performed for each stage . As the control, 3-6 replicates were performed for each stage of wild type samples
 
Contributor(s) Liu X, Gao Y, Wang C, liu W, Li J, Liu W, Cai T, Zhang Y, Gao S
Citation(s) 27462457
Submission date Jul 07, 2015
Last update date May 15, 2019
Contact name Shaorong Gao
Organization name Tongji University
Department School of life science and technology
Lab Gaolab
Street address 1239 Siping Road, Yangpu District
City Shanghai
State/province Shanghai
ZIP/Postal code 200092
Country China
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (145)
GSM1811625 NT 2-cell arrest 1 [RNA-seq]
GSM1811626 NT 2-cell arrest 2 [RNA-seq]
GSM1811627 NT 2-cell arrest 3 [RNA-seq]
This SubSeries is part of SuperSeries:
GSE70608 Single Cell Sequencing Identifies Key Epigenetic Regulators in Nuclear Transfer Mediated Reprogramming.
Relations
BioProject PRJNA289146
SRA SRP060478

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Supplementary file Size Download File type/resource
GSE70605_RAW.tar 112.8 Mb (http)(custom) TAR (of FPKM_TRACKING)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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