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| Status |
Public on Jul 03, 2015 |
| Title |
IL-10+ T cells compared to IFNg+/IL-10- T cells induced by IL-10 tolerogenic dendritic cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Ex vivo generated tolerogenic dendritic cells (tDCs) have a strong therapeutic potential to induce antigen-specific iTreg upon infusion in patients. We previously demonstrated that IL-10 tDC-primed T cells are very suppressive and produce IL-10. Here, we show that the majority of the IL-10+ T cells co-express IFNγ, giving rise to the question whether these cells are proinflammatory or regulatory. Whole genome gene expression analysis revealed a strong regulatory gene profile and a suppressed Th1 gene profile for the IL-10/ IFNγ co-expressing CD4+ T cells.
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| Overall design |
Naïve CD4+ T cells were co-cultured for 13 days with allogeneic IL-10 dendritic cells in the presence of anti-TNFα agents. After 13 days, T cells were restimulated with PMA and ionomycin. The total IL-10+ T cell population and the IFNγ+/ IL-10- T cell population were isolated with an IL-10/ IFNγ combined cytokine secretion assay and subsequent FACS sorting. Micro arrays were performed on samples of three independent donors.
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| Contributor(s) |
Boks MA, Kager-Groenland JR, van Ham SM, ten Brinke A |
| Citation(s) |
26639194 |
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| Submission date |
Jul 02, 2015 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Anja ten Brinke |
| E-mail(s) |
a.tenbrinke@sanquin.nl
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| Phone |
+31-20-5123158
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| Organization name |
Sanquin Blood Supply Foundation
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| Department |
Immunopathology
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| Street address |
Plesmanlaan 125
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| City |
Amsterdam |
| ZIP/Postal code |
1066 CX |
| Country |
Netherlands |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA288800 |
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