|
| Status |
Public on Jun 30, 2016 |
| Title |
RNA-sequencing analysis of liver from mice overexpressing miR-30c |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
This study aims to investigate the role of microRNA-30c on hepatic and metabolic gene expression and physiology
|
| |
|
| Overall design |
For this experiment, we used male C57BL/6 mice. At an age of 8 weeks, we started them on Western diet for one month and then injected them with either PBS or increasing dose of Scr or miR-30c mimic (2.5, 5.0, and 7.5 mg/kg) for 6 weeks. Liver from these mice were harvested and flash frozen. RNA from the livers of these mice were extracted and RNA-seq was performed.
|
| |
|
| Contributor(s) |
Peck BC, Sethupathy P, Hussain M, Irani S, Pan X, Iqbal J |
| Citation(s) |
27365390 |
| |
| Submission date |
Jun 28, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Bailey Peck |
| E-mail(s) |
bpeck@email.unc.edu
|
| Organization name |
University of North Carolina at Chapel Hill
|
| Department |
Genetics
|
| Lab |
Sethupathy
|
| Street address |
120 Mason Farm Rd, 5100H Genetic Medicine Bldg
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (11)
|
|
| Relations |
| BioProject |
PRJNA288386 |
| SRA |
SRP059938 |
Less...
More...