|
| Status |
Public on Jun 27, 2015 |
| Title |
Oncogene-dependent regulation of tumor cells via stromal reciprocal signalling |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Cancer is a heterocellular disease composed of tumor cells and stromal cells. Although stromal cells are known to regulate cancer progression, oncogene-dependent signalling through heterocellular cancer systems remains poorly elucidated. Here, we describe KRASG12D-dependent ‘reciprocal’ signalling across tumor and stromal Pancreatic Ductal Adenocarcinoma (PDA) cells. Heterocellular multivariate phosphoproteomics demonstrates how an oncogenic cue (KRASG12D), a trans-cellular signal (SHH), and stromal cells drive a reciprocal response in tumor cells. KRASG12D-dependent reciprocal signalling regulates the tumor cell phosphoproteome, total proteome, and mitochondria activity via an IGFR1/AXL-AKT axis. The reciprocal KRASG12D signalling state requires a heterocellular context and is unreachable by cell-autonomous oncogenic KRAS alone. These findings provide evidence that oncogenic KRAS regulates tumor cells via heterocellular reciprocation.
|
| |
|
| Overall design |
Comparison between FACS resolved iKRAS cells (previously in co-culture with PSCs) pertubed with a SHH antibody
|
| |
|
| Contributor(s) |
Tape CJ, Ling S, Worboys JD, Leong HS, McMahon KM, Norrie IC, Miller CJ, Lauffenburger DA, Jørgensen C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jun 27, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Hui Sun Leong |
| E-mail(s) |
HuiSun.Leong@cruk.manchester.ac.uk
|
| Organization name |
Cancer Research UK Manchester Institute
|
| Department |
The University of Manchester
|
| Lab |
Computational Biology Support Team
|
| Street address |
Wilmslow Road
|
| City |
Manchester |
| ZIP/Postal code |
M20 4BX |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA288372 |
| SRA |
SRP059937 |
Less...
More...