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Series GSE7021 Query DataSets for GSE7021
Status Public on Feb 17, 2007
Title Chorionic villi cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary In the chorionic villi of placenta, trophoblasts and endothelial cells are present, and moreover mesenchymal cells (stromal cells) can be obtained. We generated cells with the mesenchymal phenotype from the chorionic mesoderm, and showed that: a) physiologically functioning cardiomyocytes were transdifferentiated from human placenta-derived chorionic villi cells, but these cells did not induce to osteoblasts and adipocytes ; b) the cardiomyogenic induction rate obtained using our system was relatively high compared to that obtained using the previously described method ; c) co-cultivation with fetal murine cardiomyocytes alone without transdifferentiation factors such as 5-azaC or oxytocin is sufficient for cardiomyogenesis in our system; d) Chorionic villi cells have the electrophysiological properties of 'working' cardiomyocytes. The chorionic mesoderm contained a large number of cells with a cardiomyogenic potential.
Keywords: Cardiomyogenic induction
Overall design To isolate chorionic villi cells, we used the explant culture method, in which the cells were outgrown from pieces of chorionic villi attached to dishes. Chorionic villi cells were harvested with 0.25% trypsin and 1 mM EDTA, and overlaid onto the cultured fetal cardiomyocytes at 7 x 103/cm2. Every 2 days, the culture medium was replaced with fresh culture medium that was supplemented with 10% FBS and 1 ug/ml Amphotericin B (GIBCO). The morphology of the beating chorionic villi cells was evaluated under a fluorescent microscope.
Citation(s) 17544394
Submission date Feb 13, 2007
Last update date Aug 10, 2018
Contact name Akihiro Umezawa
Phone 81-3-5494-7047
Organization name National Center for Child Health and Development
Department Center for Regenerative Medicine
Street address 2-10-1 Okura
City Setagaya-ku
State/province Tokyo
ZIP/Postal code 157-8535
Country Japan
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (2)
GSM162104 CVCs, undifferentiated at growing phase
GSM162105 CVCs, differentiated at growing phase
BioProject PRJNA98369

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7021_RAW.tar 7.5 Mb (http)(custom) TAR (of CEL, EXP)

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