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Series GSE70123 Query DataSets for GSE70123
Status Public on Jun 30, 2016
Title Adipose Rab10 Knockout Causes Insulin Resistance through Impaired Glucose Uptake
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Insulin action in adipocytes affects whole-body insulin sensitivity. Studies of adipose-specific Glut4 knockout mice have established that adipose Glut4 contributes to the control of systemic glucose homeostasis. Presumably, this reflects a role for Glut4-mediated glucose transport in the regulation of secreted adipokines. In cultured 3T3-L1 adipocytes, Rab10 GTPase is required for insulin-stimulated translocation of Glut4 (Sano et al., 2007). The physiological importance of adipose Rab10 and the significance of its role in the control of Glut4 vesicle trafficking in vivo are unknown. Here we report that adipocytes from adipose-specific Rab10 knockout mice have a ~50% reduction in glucose uptake and Glut4 translocation to the cell surface in response to insulin, demonstrating a role for Rab10 in Glut4 trafficking. Moreover, hyperinsulinemic-euglycemic clamp shows decreased whole-body glucose uptake as well as impaired suppression of hepatic glucose production in adipose Rab10 knockout mice. Thus, fully functional Glut4 vesicle trafficking in adipocytes is critical for maintaining insulin sensitivity. Comparative transcriptome analysis of perigonadal adipose tissue demonstrates significant transcriptional similarities between adipose Rab10 knockout mice and adipose Glut4 knockout mice, consistent with the notion that the phenotypic similarities between the two models are mediated by reduced insulin-stimulated glucose transport into adipocytes.
Overall design Transcriptome sequencing of perigonadal white adipose tissue
Contributor(s) Vazirani R, McGraw TE, Elemento O, Verma A
Citation(s) 27207531
Submission date Jun 22, 2015
Last update date May 15, 2019
Contact name Akanksha Verma
Organization name Weill Cornell Medical College
Department Department of Physiology and Biophysics
Street address 1300, York Avenue
City New York
State/province New York
ZIP/Postal code 10021
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (6)
GSM1717324 FLOX 282
GSM1717325 AR10KO 302
GSM1717326 AR10KO 341
BioProject PRJNA287703
SRA SRP059753

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Supplementary file Size Download File type/resource
GSE70123_RAW.tar 650.0 Kb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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