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| Status |
Public on Sep 20, 2017 |
| Title |
Large scale sequencing of PSTVd-I sRNA in tomato plants |
| Organism |
Potato spindle tuber viroid |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
In order to analyze the production of small RNA (sRNA) by Potato spindle tuber viroid-intermediate strain (PSTVd-I) upon infecting the plants, the tomato plants (Lycopersicum esculentum cv. Rutgers) were inoculated with the PSTVd-I. After 21-days of post inoculation, total RNA was extracted and subjected for deep-sequencing using Illumina MiSeq platform. The primers were trimmed and the 21- to 24-nt long small RNA species were filtered after quality check of the raw data.
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| |
|
| Overall design |
Examination for the production of viroid specific small RNA by PSTVd upon infection in tomato plants.
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| |
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| Contributor(s) |
Adkar-Purushothama CR, Perreault J |
| Citation(s) |
28827569 |
| |
| Submission date |
Jun 22, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Charith Raj Adkar-Purushothama |
| E-mail(s) |
charith.adkar@usherbrooke.ca
|
| Organization name |
Université de Sherbrooke
|
| Department |
Biochemistry
|
| Street address |
3201 rue Jean-Mignault
|
| City |
Sherbrooke |
| State/province |
Quebec |
| ZIP/Postal code |
J1E 4K8 |
| Country |
Canada |
| |
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| Platforms (1) |
| GPL20595 |
Illumina MiSeq (Potato spindle tuber viroid) |
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| Samples (1) |
|
| Relations |
| BioProject |
PRJNA287631 |
| SRA |
SRP059727 |
