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Series GSE69982 Query DataSets for GSE69982
Status Public on Mar 24, 2016
Title Primary cilium-autophagy-Nrf2 (PAN) axis links the cell cycle to neuroectoderm fate in human embryonic stem cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Under defined differentiation conditions human embryonic stem cells (hESCs) can be directed toward a mesendodermal (ME) or neuroectoderm (NE) fate, the first decision during hESC differentiation. Coupled with G1 lengthening a divergent ciliation pattern emerged within the first 24 hours of induced lineage specification and these changes heralded a neuroectoderm decision before any neural precursor markers were expressed. By day 2, increased ciliation in NE precursors induced autophagy that resulted in the inactivation of Nrf2. Nrf2 binds directly to upstream regions of the OCT4 and NANOG genes to promote their expression and represses NE derivation. Nrf2 suppression was sufficient to rescue poorly neurogenic iPSC lines. Only after these events have been initiated do neural precursor markers get expressed at day 4. Thus we have identified a primary cilium-autophagy-Nrf2 (PAN) axis coupled to cell cycle progression that directs hESCs toward NE.
Overall design Transcriptome analysis of hESC-derived neuroectoderm and mesendoderm cells
Contributor(s) Jang J, Wang Y, Lalli MA, Guzman E, Godshalk SE, Zhou H, Kosik KS
Citation(s) 27020754
Submission date Jun 18, 2015
Last update date May 15, 2019
Contact name Kenneth S. Kosik
Organization name UCSB
Department NRI
Lab Kosik
Street address UC Santa Barbara
City Santa Barbara
State/province California
ZIP/Postal code 93106
Country USA
Platforms (1)
GPL17303 Ion Torrent Proton (Homo sapiens)
Samples (9)
GSM1715067 H9 hESC rep1
GSM1715068 H9 hESC rep2
GSM1715069 H9 hESC rep3
BioProject PRJNA287325
SRA SRP059624

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Supplementary file Size Download File type/resource
GSE69982_refSeq.rpkm.gz 844.5 Kb (ftp)(http) RPKM
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Processed data are available on Series record

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