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| Status |
Public on Jun 18, 2015 |
| Title |
Poised chromatin and bivalent domains facilitate the mitosis-to-meiosis transition in the male germline |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The male germline transcriptome changes dramatically during the mitosis-to-meiosis transition to activate late spermatogenesis genes and to transiently suppress genes commonly expressed in somatic lineages and spermatogenesis progenitor cells, termed somatic/progenitor genes.
These changes reflect epigenetic regulation. Induction of late spermatogenesis genes during spermatogenesis is facilitated by poised chromatin established in the stem cell phases of spermatogonia, whereas silencing of somatic/progenitor genes during meiosis and postmeiosis is associated with formation of bivalent domains which also allows the recovery of the somatic/progenitor program after fertilization. Importantly, during spermatogenesis mechanisms of epigenetic regulation on sex chromosomes are different from autosomes: X-linked somatic/progenitor genes are suppressed by meiotic sex chromosome inactivation without deposition of H3K27me3. Our results suggest that bivalent H3K27me3 and H3K4me2/3 domains are not limited to developmental promoters (which maintain bivalent domains that are silent throughout the reproductive cycle), but also underlie reversible silencing of somatic/progenitor genes during the mitosis-to-meiosis transition in late spermatogenesis.
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| |
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| Overall design |
29 samples analyzed by ChIP-Seq
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| |
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| Contributor(s) |
Hosu S, Kartashov AV, Hasegawa K, Barski A, Namekawa SH |
| Citation(s) |
26198001 |
| |
| Submission date |
Jun 17, 2015 |
| Last update date |
Oct 10, 2019 |
| Contact name |
Satoshi Namekawa |
| E-mail(s) |
satoshi.namekawa@cchmc.org
|
| Phone |
513-803-1377
|
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Street address |
3333 Burnet Ave
|
| City |
Cincinnati |
| State/province |
Ohio |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (29)
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| Relations |
| BioProject |
PRJNA287221 |
| SRA |
SRP059590 |
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