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Series GSE69908 Query DataSets for GSE69908
Status Public on Sep 29, 2015
Title Gene Expression Profiling of Murine Melanomas Exhibiting Differential Tumorigenicities
Organism Mus musculus
Experiment type Expression profiling by array
Summary The goal of this study was to compare the gene expression profiles of a highly versus poorly tumorigenic murine melanoma. B16-F1 is a well-characterized murine melanoma cell line that grows progressively in hosts, both as primary tumors in subcutaneous tissue and as metastatic lesions in internal organs. D5.1G4 is a chemically mutated variant of B16 melanoma that grows with significantly slower kinetics than its wild-type counterpart. Whole genome expression microarray analysis of RNA isolated from these murine melanoma lines was performed to provide insights into factors that regulate the growth and metastasis of these tumors, which are useful models for investigating in a murine system the outgrowth, progression, and control of a cancer type that is prevalent in humans worldwide.
Overall design B16-F1 or D5.1G4 melanoma cells were cultured for 24 hours before isolating RNA for whole genome expression analysis using the Agilent Mouse 4x44K Gene Expression Microarray v2 (Agilent Design ID 026655). Samples were run in replicates of three. D5.1G4 melanoma served as the reference sample, and genes expressed at 2-fold higher or lower levels in B16-F1 (with a p value ≤ 0.05) were defined as genes exhibiting differential expression between the two tumors with statistical significance.
Contributor(s) Hargadon KM
Citation(s) 26441959
Submission date Jun 16, 2015
Last update date Nov 01, 2017
Contact name Kristian Michael Hargadon
Phone 434-223-6261
Organization name Hampden-Sydney College
Department Biology
Lab Hargadon Lab
Street address Brown Student Center, Box 837
City Hampden-Sydney
State/province VA
ZIP/Postal code 23943
Country USA
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (6)
GSM1712347 B16-F1_melanoma_rep1
GSM1712348 B16-F1_melanoma_rep2
GSM1712349 B16-F1_melanoma_rep3
BioProject PRJNA287114

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Supplementary file Size Download File type/resource
GSE69908_RAW.tar 12.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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