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| Status |
Public on Mar 24, 2016 |
| Title |
Atad2 is a generalist facilitator of chromatin dynamics in embryonic stem cells [Microarray transcriptomic analysis] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Although the conserved AAA ATPase – bromodomain factor, ATAD2, has been described as a transcriptional co-activator upregulated in many cancers, its function remains poorly understood. Here, using a combination of ChIP-seq, ChIP-proteomics and RNA-seq experiments in embryonic stem cells, we found that Atad2 is an abundant nucleosome-bound protein present on active genes, associated with chromatin remodelling, DNA replication and DNA repair factors. A structural analysis of its bromodomain and subsequent investigations demonstrate that histone acetylation guides ATAD2 to chromatin, resulting in an overall increase of chromatin accessibility and histone dynamics, which is required for the proper activity of the highly expressed gene fraction of the genome. While in exponentially growing cells Atad2 appears dispensable for cell growth, in differentiating ES cells, Atad2 becomes critical in sustaining specific gene expression programs, controlling proliferation and differentiation. Altogether, this work defines Atad2’s function as a facilitator of general chromatin-templated activities such as transcription.
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| Overall design |
Transcriptomic analyses comparing ES cells stably expressing anti-Atad2 shRNA versus cells expressing control shRNA, in day 3 LIF (-) differentiating ES cells (3 replicates for each condition) were performed using the Illumina MouseWG-6 v2.0 expression beadchip technology
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| Contributor(s) |
Rousseaux S, Debernardi A, Morozumi Y, Boussouar F, Guardiola P, Gerard M, Gianni D, Schweifer N, Khochbin S |
| Citation(s) |
26459632 |
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| Submission date |
Jun 15, 2015 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Sophie Rousseaux |
| Organization name |
INSERM-UGA
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| Department |
IAB
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| Lab |
U1209
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| Street address |
Bd de la Chantourne
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| City |
La Tronche |
| ZIP/Postal code |
38706 |
| Country |
France |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (6)
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| GSM1712160 |
Differentiating ES cells sh Atad2 replicate 1 |
| GSM1712161 |
Differentiating ES cells sh Atad2 replicate 2 |
| GSM1712162 |
Differentiating ES cells sh Atad2 replicate 3 |
| GSM1712163 |
Differentiating ES cells sh Ctrl replicate 1 |
| GSM1712164 |
Differentiating ES cells sh Ctrl replicate 2 |
| GSM1712165 |
Differentiating ES cells sh Ctrl replicate 3 |
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| This SubSeries is part of SuperSeries: |
| GSE70315 |
Atad2 is a generalist facilitator of chromatin dynamics in embryonic stem cells |
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| Relations |
| BioProject |
PRJNA287043 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE69899_RAW.tar |
15.8 Mb |
(http)(custom) |
TAR |
| GSE69899_non-normalized.txt.gz |
1.8 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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