|
| Status |
Public on Apr 27, 2007 |
| Title |
Spermatozoal RNA Profiles (Human-6 BeadChip) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Normal human spermatogenesis concludes with the formation of large numbers of morphologically well developed spermatozoa. While transcriptionally quiescent these cells carry an RNA payload that reflects the final spermiogenic phase of transcription. We report here the spermatozoal transcript profiles characteristic of normally fertile individuals and infertile males suffering from a consistent and severe teratozoospermia in which under 4% of spermatozoa are morphologically normal. RNA was extracted from the purified sperm cells of ejaculate and hybridized to Illumina WG6 Microarrays.
Keywords: disease state analysis
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| Overall design |
Spermatozoal RNAs were prepared from the semen samples of 13 individuals. An asymmetric dual block design was adopted with biological replicates in both blocks. 5 semen samples were assessed from normally fertile males who had fathered at least one child. 8 semen samples were assessed from infertile individuals with a severe and consistent heterogeneous teratozoospermia who showed no other abnormal semen parameters.
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| Web link |
http://compbio.med.wayne.edu
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| |
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| Contributor(s) |
Platts AE, Dix DJ, Chemes HE, Thompson KE, Goodrich R, Rockett JC, Rawe VY, Quintana S, Diamond MP, Strader LF, Krawetz SA |
| Citation(s) |
17327269 |
| |
| Submission date |
Feb 06, 2007 |
| Last update date |
Mar 17, 2012 |
| Contact name |
Stephen A Krawetz |
| E-mail(s) |
steve@compbio.med.wayne.edu
|
| Organization name |
Wayne State University
|
| Street address |
275 East Hancock, CS Mott Center
|
| City |
Detroit |
| State/province |
MI |
| ZIP/Postal code |
48207 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL2507 |
Sentrix Human-6 Expression BeadChip |
|
| Samples (13)
|
| GSM160620 |
Teratozoospermic individual: Sample T2 |
| GSM160621 |
Teratozoospermic individual: Sample T1 |
| GSM160622 |
Teratozoospermic individual: Sample T6 |
| GSM160623 |
Teratozoospermic individual: Sample T4 |
| GSM160624 |
Teratozoospermic individual: Sample T8 |
| GSM160625 |
Normospermic individual: Sample N11 |
| GSM160626 |
Teratozoospermic individual: Sample T3 |
| GSM160627 |
Teratozoospermic individual: Sample T7 |
| GSM160628 |
Teratozoospermic individual: Sample T5 |
| GSM160629 |
Normospermic individual: Sample N6 |
| GSM160630 |
Normospermic individual: Sample N12 |
| GSM160631 |
Normospermic individual: Sample N5 |
| GSM160632 |
Normospermic individual: Sample N1 |
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| This SubSeries is part of SuperSeries: |
| GSE6969 |
Spermatozoal RNA Profiles from Normally Fertile and Heterogeneously Teratozoospermic Subjects |
|
| Relations |
| BioProject |
PRJNA104135 |
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