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| Status |
Public on Jun 06, 2015 |
| Title |
Effect of high-fat diet (HFD) on gene expression in brown adipose tissue (BAT) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
C57BL/6 male mice were fed a standardized NCD or HFD after weaning (4 weeks of age) over a course of 12 weeks.
Normal chow diet (NCD) fed animals served as Controls
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| Overall design |
Biotin-labeled cDNA was synthesized using GeneChip Whole Transcript Sense Labeling Assay (Affymetrix) according to vendor’s instructions. After fragmentation, cDNAs were hybridized for 17h at 45°C on Affymetrix Mouse Gene 1.0 ST Arrays. The Arrays were washed and stained in the GeneChip Fluidics Station 450 and scanned on a GeneChip Scanner 3000 7G (Affymetrix). Data intensities were log transformed and normalized with a quantile normalization method using Affymetrix Power Tools. Differentially expressed genes were identified according to statistical evidence indicated by Student's t-test and fold change statistics
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| Contributor(s) |
Brüning JC, Kornfeld J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jun 05, 2015 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Jan-Wilhelm Kornfeld |
| E-mail(s) |
janwilhelmkornfeld@bmb.sdu.dk
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| Phone |
004593507481
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| Organization name |
University of Southern Denmark
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| Lab |
Noncoding RNA and Energy Homeostasis
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| Street address |
Campusvej 55
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| City |
Odense |
| State/province |
Fyn |
| ZIP/Postal code |
5230 |
| Country |
Denmark |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA285964 |
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