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Series GSE69596 Query DataSets for GSE69596
Status Public on Dec 31, 2015
Title Gene expression analysis of CD4+ and CD4- ILC1 subsets by RNAseq
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Innate lymphoid cell (ILC) subsets that mirror helper T cells in their effector cytokine profiles have recently emerged as central players in both homeostatic and inflammatory conditions. Like their Th1, Th2 and Th17/Th22 helper T cell counterparts, ILC subsets are categorized based on their expression of specific transcription factors and effector cytokines: group 1 ILC (ILC1) express T-bet and IFN-γ; group 2 ILC (ILC2) express GATA-3 and type 2 effector cytokines such as IL-13 and IL-5; and group 3 ILC (ILC3) express RORgt and the cytokines IL-22 and/or IL-17. Under this nomenclature, natural killer (NK) cells and lymphoid tissue inducers (LTi) are considered ILC1 and ILC3, respectively. ILC1 contain both CD4+ and CD4- populations, but whether this phenotypic characteristic reflects functional differences between these two populations is unknown. These studies examine the gene expression profiles of CD4+ vs CD4- ILC1 in a cohort of healthy control subjects.
Overall design ILC subsets were isolated from the peripheral blood of healthy control subjects. cDNA was isolated and amplified from sorted populations, and gene expression was analyzed by RNAseq
Contributor(s) Roan F, Stoklasek TA, Whalen E, Bluestone JA, Mason M, Presnell S, Ziegler SF
Citation(s) 26826243
Submission date Jun 05, 2015
Last update date May 15, 2019
Contact name Scott Presnell
Organization name Benaroya Research Institute
Street address 1201 Ninth Avenue
City Seattle
State/province WA
ZIP/Postal code 98101
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (8)
GSM1704298 1_CD4+ ILC1
GSM1704299 2_CD4- ILC1
GSM1704300 3_CD4+ ILC1
BioProject PRJNA285954
SRA SRP059170

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Supplementary file Size Download File type/resource
GSE69596_ILC1rawCounts.csv.gz 277.0 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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