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| Status |
Public on Jul 01, 2015 |
| Title |
Gene expression profiling of BDC2.5 CD4T cells isolated from NOD mice after in vivo antigen stimulation with either DEC205+ or DCIR2+ DCs. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We identified DCIR2+DCs but not DEC205+DCs as able to induce peripheral T cell tolerance in pre-diabetic autoimmune NOD mice. To determine what distinct genetic programs are elicited in the auto-reactive CD4 T cells early after stimulation by these two DC subsets, we utilized adoptive transfer of BDC2.5 CD4 T cells into NOD mice, which were then given chimeric antibody to deliver the beta-cell specific antigen to either DCIR2+DCs or DEC205+DCs, leading to BDC2.5 CD4 T cell specific stimulation in vivo. The analysis shows that the negative transcriptional factor Zbtb32 (ROG) is up-regulated more in BDC2.5 CD4 T cells after stimulated with a antigen via DCIR2+DCs presentation, compared with DEC205+DCs, suggesting the involvement of Zbtb32 in DCIR2+DCs-mediated auto-reactive T cell tolerance in disease ongoing NOD mice.
The BDC2.5 CD4 T cells after 14 hour of injection with a 100 ng of anti-DCIR2-BDC, anti-DEC205-BDC antibody, or PBS as a control to NOD mice, were sorted by BD FACSAria and their expression profile was analyzed using Affymetrix chips.
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| Overall design |
NOD mice with transferred BDC2.5 T cell were injected with 100 ng of anti-DCIR2-BDC (NOD_DCIR2-BDC), anti-DEC205-BDC antibody (NOD_DEC-BDC), or PBS (NOD_PBS) as a control. After 14 hours, BDC2.5 CD4 T cells were sorted by BD FACSAria and their expression profile was analyzed using Affymetrix microarray chips. Each sample has a biological duplicate.
Raw data were preprocessed with the RMA algorithm in Partek, and averaged expression values were used for analysis.
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| Contributor(s) |
Price JD, Hotta-Iwamura C, Zhao Y, Beauchamp NM, Tarbell KV |
| Citation(s) |
26070317 |
| |
| Submission date |
Jun 04, 2015 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Kristin V. Tarbell |
| E-mail(s) |
tarbellk@niddk.nih.gov
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| Organization name |
National Institute of Diabetes and Digestive and Kidney Diseases
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| Department |
Diabetes, Endocrinology, and Obesity Branch
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| Lab |
Immune Tolerance Section
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| Street address |
Bldg.10, CRC, 5W-5940, 10 Center Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA285843 |
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