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| Status |
Public on Jul 31, 2015 |
| Title |
Gene expression profile of A549 cells cultured with novel 3D cell culture method |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Preclinical cancer drug discovery efforts have employed two-dimensional (2D)-cell-based assay models, which fail to forecast in vivo efficacy and contribute to a lower success rates of clinical approval. Three-dimensional (3D) cell culture models are recently expected to bridge the gap between 2D and in vivo models. We have developed novel 3D culture method that improves the growth of spheroid-forming cancer cells under anchorage-independent condition by leveraging a feature of FP001.
Gene microarrays were used to observe the global gene expression in A549 cells cultured with normal adhesion plate (2D, control) or with low adhesion plate (+FP001) and identified distinct classes of up or down-regulated genes.
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| Overall design |
A549 cells were cultured for 5 days in three different conditions as follows. (1) Normal attachment plates with normal medium (as control), (2) low-attachment plates with normal medium, (3) low-attachment plates with FP001 containing medium. Each sample was collected three times.
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| Contributor(s) |
Aihara A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jun 02, 2015 |
| Last update date |
Mar 15, 2019 |
| Contact name |
Ayako Otani |
| E-mail(s) |
otania@nissanchem.co.jp
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| Organization name |
Nissan Chemical Industries
|
| Department |
Medical Materials group
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| Street address |
1470 Shiraoka
|
| City |
Shiraoka |
| State/province |
Saitama |
| ZIP/Postal code |
349-0294 |
| Country |
Japan |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA285682 |
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