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Series GSE69476 Query DataSets for GSE69476
Status Public on Feb 02, 2016
Title mRNA sequencing of the global effect of SOX2 on gene expression in hESC and hESC derived NPCs.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Global transcriptome analysis reveals that SOX2 regulates a common group safeguarding stem cell identity in hESCs and hNPCs, and also distinct functional groups regulating diverse cell type dependent developmental processes in hESCs and hNPCs, sheding light on the mechanism underlying SOX2's dynamic function in the two related stem cell types.
Overall design To analyze the global effect of SOX2 on gene expression in hESC and hNPC, we analyzed the gene expression profiles upon SOX2 KD in hESC and hNPC. Due to the compensation effect of SOX3 in hESC, we used non-targeting siRNA (siNT) and two sets of siRNAs targeting both SOX2 and SOX3 (siSOX2/3-1 and siSOX2/3-2) to knock down both SOX2 and SOX3 in hESC and compared the gene expression profile between siNT and siSOX2/3 by RNA-seq. We also compared the gene expression profiles in hNPC treated with siNT and two SOX2 siRNAs (siSOX2-1 and siSOX2-2) by RNA-seq.
Contributor(s) Zhou C, Jin Y
Citation(s) 26809499
Submission date Jun 02, 2015
Last update date May 15, 2019
Contact name Ying Jin
Organization name Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
Street address 225 South Chongqing Road
City Shanghai
ZIP/Postal code 200025
Country China
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (6)
GSM1701810 hESC NT
GSM1701811 hESC SOX2/3 KD1
GSM1701812 hESC SOX2/3 KD2
BioProject PRJNA285790
SRA SRP059033

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Supplementary file Size Download File type/resource
GSE69476_RAW.tar 1.3 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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