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| Status |
Public on May 28, 2016 |
| Title |
Transcriptome and translatome landscape of Streptomyces coelicolor M145 |
| Organism |
Streptomyces coelicolor |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
We have integrated nucleotide resolution genome-scale measurements of the transcriptome and translatome of the Streptomyces coelicolor A3(2), the model antibiotic-producing actinomycete. Our systematic study determined 3,473 transcription start sites, leading to discovery of a high proportion (~21%) of leaderless mRNAs and 230 non-coding RNAs; this enabled deduction of promoter architecture on a genome-scale. Ribosome profiling analysis revealed that the translation efficiency was negatively correlated for secondary metabolic genes. These results provide novel fundamental insights into translational regulation of secondary metabolism that enables rational synthetic biology approaches to awaken such ‘silent’ secondary metabolic pathways.
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| Overall design |
Profiles of primary transcripts, whole transcripts, and ribosome protected fragments (RPFs) of Streptomyces coelicolor were generated by deep sequencing using Illumina Miseq.
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| Contributor(s) |
Jeong Y, Kim J, Kim M, Bucca G, Cho S, Yoon Y, Kim B, Kim S, Roe J, Smith CP, Cho B |
| Citation(s) |
27251447 |
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| Submission date |
May 28, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Yujin Jeong |
| E-mail(s) |
dbwls54@gmail.com
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| Organization name |
KAIST
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| Street address |
291 Daehak-ro
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| City |
Daejeon |
| ZIP/Postal code |
34141 |
| Country |
South Korea |
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| Platforms (1) |
| GPL20259 |
Illumina MiSeq (Streptomyces coelicolor) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA285265 |
| SRA |
SRP058830 |
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