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| Status |
Public on Aug 31, 2015 |
| Title |
Analysis of gene expression changes in KPT-185-treated HMLE cells transduced to stably overexpress SNAI1 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
HMLE-SNAIL cell cultures were treated with KPT-185 drug (KPT, 150 nanomolar) or vehicle (DMSO) for 24 hours, in parallel quadruplicates. KPT-185 is an Exportin 1 (XPO1) inhibitor with possible pluripotent or unexpected effects on cell signaling.
Total RNA was isolated and analyzed in an Agilent two-color experiment, where four biological replicates of each condition were directly compared, expression ratios are KPT-treated condition relative to control (DMSO vehicle-treated).
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| Overall design |
The experiment object was to identify genes that displayed significantly altered expression in response to KPT treatment inorder to confirm on-target drug impact and elucidate other potential effects in this cell line model.
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| Contributor(s) |
Azmi A, Bollig-Fischer A |
| Citation(s) |
26536918 |
| |
| Submission date |
May 28, 2015 |
| Last update date |
Jun 30, 2017 |
| Contact name |
Aliccia Bollig-Fischer |
| E-mail(s) |
bollig@karmanos.org
|
| Organization name |
Karmanos Cancer Institute & Wayne State University
|
| Department |
Oncology
|
| Street address |
4100 John R
|
| City |
Detroit |
| ZIP/Postal code |
48201 |
| Country |
USA |
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| Platforms (1) |
| GPL10332 |
Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Feature Number version) |
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| Samples (4)
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| GSM1697869 |
HMLE-SNAIL cell line KPT-treated relative to vehicle-treated, replicate 1 of 4 |
| GSM1697870 |
HMLE-SNAIL cell line KPT-treated relative to vehicle-treated, replicate 2 of 4 |
| GSM1697871 |
HMLE-SNAIL cell line KPT-treated relative to vehicle-treated, replicate 3 of 4 |
| GSM1697872 |
HMLE-SNAIL cell line KPT-treated relative to vehicle-treated, replicate 4 of 4 |
|
| Relations |
| BioProject |
PRJNA285194 |
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