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| Status |
Public on May 28, 2015 |
| Title |
Transcriptome data from induced Pluripotent Stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We have generated human induced Pluripotent Stem cells (hiPSc) using Sendai virus-mediated delivery of reprogramming factors. hiPSc lines have been screened using SNP array to assess chromosomal stability (alongside the fibroblast lines from which they derived), and validation of the pluripotency of the hiPSc lines is provided by Pluritest assessment of transcriptome datasets, prior to differentiation and downstream assays.
Single cell RT-qPCR analysis of induced pluripotent stem cell-derived cortical neurons reveals frequent dual layer identity. Handel AE, Chintawar S, Lalic T, Whiteley E, Giustacchini A, Argoud K, Sopp P, Bowden R, Cowley SA, Newey S, Ackerman C, Ponting CP and Cader ZM. Submitted
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| Overall design |
human iPSc lines were derived from human dermal fibroblasts from control donors. SNP and gene expression datasets from control lines. Note that iPS NHDF-1, also used in the current study, has been published previously [Van Wilgenburg B., Browne C., Vowles J. & Cowley S. A. Efficient, long term production of monocyte-derived macrophages from human pluripotent stem cells under partly-defined and fully-defined conditions. PLoS One 8, e71098 (2013); Physiological characterisation of human iPS-derived dopaminergic neurons. Hartfield EM, Yamasaki-Mann M, Ribeiro Fernandes HJ, Vowles J, James WS, Cowley SA, Wade-Martins R. PLoS One. 2014 9(2):e87388]
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| Contributor(s) |
Handel AE, Chintawar S, Lalic T, Whiteley E, Giustacchini A, Argoud K, Sopp P, Bowden R, Cowley SA, Newey S, Ackerman C, Ponting CP, Cader ZM |
| Citation(s) |
26740550 |
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| Submission date |
May 27, 2015 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Sally A Cowley |
| E-mail(s) |
sally.cowley@path.ox.ac.uk
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| Phone |
+44 (0) 1865 275600
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| Organization name |
University of Oxford
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| Department |
Sir William Dunn School of Pathology
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| Lab |
James Martin Stem Cell Facility
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| Street address |
South Parks Road
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| City |
Oxford |
| ZIP/Postal code |
OX1 3RE |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE69302 |
SNP data from AH017 human fibroblasts and induced Pluripotent Stem cells |
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| Relations |
| BioProject |
PRJNA285110 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE69288_RAW.tar |
26.2 Mb |
(http)(custom) |
TAR |
| GSE69288_non-normalized.txt.gz |
822.3 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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