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Series GSE69099 Query DataSets for GSE69099
Status Public on Feb 26, 2016
Title Comprehensive Epigenomic Analysis Reveals Dynamic Regulatory Programs Of Blood Development (TF ChIP-seq)
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Embryonic hematopoiesis is regulated by the coordinated interaction between transcription factors and the epigenetic regulators driving developmental-stage specific gene expression but how this process drives hematopoietic specification and terminal differentiation is poorly understood. Here we generated RNA-Seq, DNase-Seq and ChIP-Seq data for histone marks and transcription factors from ES-cell derived purified cells representing six sequential stages of blood cell specification and differentiation. Our data reveal the binding patterns of specific transcription factors involved in the priming and maintenance of distal elements and inform how binding impacts on promoter activity. Functional studies based on these data uncovered a previously unrecognised role for Hippo signalling in mammalian hematopoietic specification. Finally, we present a dynamic core regulatory network model for hematopoiesis and demonstrate its utility for the design of reprogramming experiments. Our study represents a powerful resource for studying hematopoiesis and demonstrates how such data can advance our understanding of mammalian development.
Overall design ChIP-seq data of histone modifications and transcription factors, and RNA-seq data obtained from purified cells representing five sequential stages of murine blood cell specification and differentiation
Contributor(s) Goode DK, Obier N, Vijayabaskar M, Lichtinger M, Lie-A-Ling M, Hannah R, Lilly AJ, Florowska M, Batta K, Challinor M, Wallace K, Assi SA, Cauchy P, Gilmour J, Hoogenkamp M, Westhead DR, Lacaud G, Kouskoff V, Göttgens B, Bonifer C
Citation(s) 26923725, 29530939
Submission date May 20, 2015
Last update date Jul 29, 2019
Contact name MS Vijayabaskar
Organization name University of Leeds
Department School of Molecular and Cellular Biology
Street address Woodhouse Lane
City Leeds
ZIP/Postal code LS2 9JT
Country United Kingdom
Platforms (3)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (29)
GSM1692840 HB_Cebpb
GSM1692841 HB_Gata2
GSM1692842 HB_Lmo2
This SubSeries is part of SuperSeries:
GSE69101 Comprehensive Epigenomic Analysis Reveals Dynamic Regulatory Programs Of Blood Development
BioProject PRJNA284539
SRA SRP058578

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Supplementary file Size Download File type/resource
GSE69099_RAW.tar 4.5 Gb (http)(custom) TAR (of BED, BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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