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Series GSE68921 Query DataSets for GSE68921
Status Public on Dec 04, 2015
Title Parkin-mediated mitophagy evokes perinatal cardiac mitochondria maturation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Exclusion of Parkin from mitochondria of perinatal cardiomyocytes interrupts structural and molecular transformations essential to normal perinatal-adult mitochondrial replacement. mRNA-sequencing from cardiac total RNA was performed at P1, P21 and 5-week stages of nontransgenic (ntg) and human-Mfn2-overexpressing (Mfn2wt) hearts, and also of tet-off control (tetoff) and human-Mfn2 T111A/S442A-overexpressing (Mfn2AA) hearts.
Overall design Libraries from all P1 samples were prepared and analyzed together, and similarly all P21 libraries, and all 5-week libraries together. To facilitate comparison across time points by accounting for batch effect, new libraries were prepared starting from total RNA from selected P21 ntg and 5-week ntg hearts subjected to prior analysis, during the same batch preparation as all P1 samples, and analyzed together. Correction for differences observed between libraries prepared from the same total RNA, but at different times, allows comparison across timepoints.
Contributor(s) Matkovich SJ, Dorn GW
Citation(s) 26785495
Submission date May 15, 2015
Last update date May 15, 2019
Contact name Scot J Matkovich
Organization name Washington University School of Medicine
Department Center for Cardiovascular Research
Street address 660 S Euclid Ave, box 8086
City St. Louis
State/province MO
ZIP/Postal code 63110
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (63)
GSM1686460 P1_Mfn2AA_10586-3
GSM1686461 P1_Mfn2AA_10586-7
GSM1686462 P1_Mfn2AA_10619-1
BioProject PRJNA284112
SRA SRP058378

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE68921_P1-P21-5wk_Mfn2AA-project_FPKM.txt.gz 4.4 Mb (ftp)(http) TXT
GSE68921_P1-P21-5wk_Mfn2AA-project_HTSeq_count.txt.gz 1.5 Mb (ftp)(http) TXT
GSE68921_genes+EnsemblGRCm38MT+hMfn2wt_cDNA.gtf.gz 4.8 Mb (ftp)(http) GTF
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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