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| Status |
Public on May 06, 2015 |
| Title |
Targets of Pax3 and Zic1 in neural crest and cranial placode progenitors |
| Organism |
Xenopus laevis |
| Experiment type |
Expression profiling by array
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| Summary |
The transcription factors Pax3 and Zic1 are among the earliest genes activated at the neural plate border. Pax3 and Zic1 in combination promote neural crest fate, while Zic1 alone regulate cranial placode progenitor formation. We used microarrays to identify the global repertoire of genes activated by these facors individually or in combination to gain insights into the molecular mechanisms underlying cell fate decision at the neural plate border.
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| Overall design |
Xenopus laevis embryos were injected at the 2-cell stage with mRNA encoding Pax3GR and Zic1GR , the hormone-inducible version of Pax3 and Zic1, alone or in combination. At the blastula stage (stage 9), animal cap explants were dissected and cultured for 8 hours in the presence of dexamethasone. A glucocorticoid receptor (GR) mRNA was also injected as negative control.
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| Contributor(s) |
Saint-Jeannet JP, Hong C, Tobias JW |
| Citation(s) |
24360908, 26101153 |
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| Submission date |
May 05, 2015 |
| Last update date |
Oct 02, 2015 |
| Contact name |
Jean-Pierre Saint-Jeannet |
| E-mail(s) |
jsj4@nyu.edu
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| Phone |
212-998-9978
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| Organization name |
New York University
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| Department |
Basic Science and Craniofacial Biology
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| Street address |
345 East 24th Street
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10010 |
| Country |
USA |
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| Platforms (1) |
| GPL10756 |
[X_laevis_2] Affymetrix Xenopus laevis Genome 2.0 Array |
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| Samples (20)
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| GSM1674941 |
Pax3GR Injected, biological rep4 |
| GSM1674942 |
Pax3GR Injected, biological rep5 |
| GSM1674943 |
Zic1GR Injected, biological rep1 |
| GSM1674944 |
Zic1GR Injected, biological rep2 |
| GSM1674945 |
Zic1GR Injected, biological rep3 |
| GSM1674946 |
Zic1GR Injected, biological rep4 |
| GSM1674947 |
Zic1GR Injected, biological rep5 |
| GSM1674948 |
Pax3GR/Zic1GR Injected, biological rep1 |
| GSM1674949 |
Pax3GR/Zic1GR Injected, biological rep2 |
| GSM1674950 |
Pax3GR/Zic1GR Injected, biological rep3 |
| GSM1674951 |
Pax3GR/Zic1GR Injected, biological rep4 |
| GSM1674952 |
Pax3GR/Zic1GR Injected, biological rep5 |
| GSM1674953 |
GR Injected, biological rep1 |
| GSM1674954 |
GR Injected, biological rep2 |
| GSM1674955 |
GR Injected, biological rep3 |
| GSM1674956 |
GR Injected, biological rep4 |
| GSM1674957 |
GR Injected, biological rep5 |
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| Relations |
| BioProject |
PRJNA283107 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE68546_RAW.tar |
72.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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