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| Status |
Public on May 31, 2015 |
| Title |
Evaluation of sub-culturing on whole genome expression of skeletal muscle-derived cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Today, cell therapy is considered as one of the most promising alternates to cure end-stage diseases. Using human cells for transplantation needs in vitro culture and passages which arises the concern of malignant transformation of cells. To evaluate the effect of sub-culturing on whole genome transcriptome of skeletal muscle-derived cells (SMDCs), microarray analysis was performed on passages 3, 6, 9 and 12 of SMDCs in three replicates.
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| Overall design |
There were 4 different passage samples (3, 6, 9, and 12) each stage with three replicates. Replicates for passage 3 were determined as control group and the data was analyzed compared to this passage.
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| Contributor(s) |
Vahdat S, Mousavi SA, Moghadasali R, Gharechahi J, Aghdami N, Salekdeh GH, Baharvand H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 04, 2015 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Seyed Ahmad Mousavi |
| E-mail(s) |
pcsam.2008@gmail.com
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| Organization name |
Royan Institute
|
| Street address |
Bani Hashem sq. - Resalat Hw.
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| City |
Tehran |
| State/province |
Tehran |
| ZIP/Postal code |
16635-148 |
| Country |
Iran |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA282931 |
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