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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 15, 2016 |
| Title |
Reconstructing divergent retinoid-induced cell fate-regulatory programs in stem cells [Affymetrix] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We have integrated dynamic RXRa binding, chromatin accessibility and promoter epigenetic status with the transcriptional activity inferred from RNA polymerase II mapping and transcription profiling. This demonstrated a temporal organization structure, in which early events are preferentially enriched for common GRNs, while cell fate specification is reflected by the activation of late programs in a cell-type specific manner. Furthermore, significant differences in cell lines' promoter status of genes associated with cell-line specific programs were inferred. Finally, a variety of transcription factors (TFs) playing a direct role in the signal transduction cascade downstream of the RXR/RAR initiated wiring were identified, several of them commonly regulated in both model systems, but in addition cell-type specific TF drivers were also identified.
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| Overall design |
time series gene expression assessed over the retinoic acide induced differentiation in P19 and F9 embryonal carcinoma cells. 5 timepoints over four different treatment conditions (ATRA; RARa agonist BMS753; RARb agonist BMS641 and the RARg agonist BMS961) assessed in P19 cells and compared with those assessed in F9 cells described in a previous study: GSE30537
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| Contributor(s) |
Mendoza-Parra M, Malysheva V, Saleem MA, Gronemeyer H |
| Citation(s) |
27650846, 36446525 |
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| Submission date |
Apr 27, 2015 |
| Last update date |
Dec 15, 2022 |
| Contact name |
Marco Antonio Mendoza-Parra |
| E-mail(s) |
marco@igbmc.fr
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| Organization name |
IGBMC
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| Street address |
1, rue Laurent Fries
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| City |
Illkirch; Strasbourg |
| ZIP/Postal code |
67400 |
| Country |
France |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (24)
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| GSM1667061 |
P19 EC cells, EtOH, rep4 |
| GSM1667062 |
P19 EC cells, ATRA, 2h |
| GSM1667063 |
P19 EC cells, ATRA, 6h |
| GSM1667064 |
P19 EC cells, ATRA, 24h |
| GSM1667065 |
P19 EC cells, ATRA, 48h |
| GSM1667066 |
P19 EC cells, ATRA, 72h |
| GSM1667067 |
P19 EC cells, BMS753, 2h |
| GSM1667068 |
P19 EC cells, BMS753, 6h |
| GSM1667069 |
P19 EC cells, BMS753, 24h |
| GSM1667070 |
P19 EC cells, BMS753, 48h |
| GSM1667071 |
P19 EC cells, BMS753, 72h |
| GSM1667072 |
P19 EC cells, BMS641, 2h |
| GSM1667073 |
P19 EC cells, BMS641, 6h |
| GSM1667074 |
P19 EC cells, BMS641, 24h |
| GSM1667075 |
P19 EC cells, BMS641, 48h |
| GSM1667076 |
P19 EC cells, BMS641, 72h |
| GSM1667077 |
P19 EC cells, BMS961, 2h |
| GSM1667078 |
P19 EC cells, BMS961, 6h |
| GSM1667079 |
P19 EC cells, BMS961, 24h |
| GSM1667080 |
P19 EC cells, BMS961, 48h |
| GSM1667081 |
P19 EC cells, BMS961, 72h |
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| This SubSeries is part of SuperSeries: |
| GSE68291 |
Reconstructing divergent retinoid-induced cell fate-regulatory programs in stem cells |
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| Relations |
| BioProject |
PRJNA283043 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE68290_RAW.tar |
111.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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