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Series GSE68283 Query DataSets for GSE68283
Status Public on Nov 02, 2015
Title Next Generation Sequencing Analysis of Wild Type and Rfx2-/- Testicular Transcriptomes [RNA-Seq P30]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: This study was carried out to determine the consequences of the Rfx2-/- genotype on spermatogenesis in the mouse
Methods: RNA was extracted from decapsulated testes of 29-30 day old mixed background mice of either genotype. Deep sequencing was used to determine quantitative expression of the genomes from independent replicates of each genotype
Results: RNA-Seq analysis identified some 640 genes that are down regulated at least 2-fold in Rfx2-/- testes, with ~150 being reduced at least 10-fold
Conclusion: Spermatogenesis undergoes complete arrest just prior to the end of the round spermatid period of sperm development in mutant mice. Sequencing results showed that approximately 640 genes were downregulated 2 fold or more in the testes of mutant mice. Comparison of similar studies of targeted mutations in genes for other transcription factor demonstrate that Rfx2 has a large and nearly unique set of genes that depend on it directly or indirectly. A large number of downregulated genes are identified with cilia function.
Overall design Testicular mRNA profiles were determined by deep sequencing using testes from 5 independent wild type and 4 independent Rfx2-/- mice
Contributor(s) S Kistler W, Baas D, Lemeille S, Paschaki M, Barras E, Ma W, Duteyrat J, Morlé L, Durand B, Reith W
Citation(s) 26162102
Submission date Apr 27, 2015
Last update date May 15, 2019
Contact name Sylvain LEMEILLE
Organization name University of Geneva Medical School
Department Department of Pathology and Immunology
Street address 1 rue Michel-Servet
City Geneva
ZIP/Postal code 1211
Country Switzerland
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (9)
GSM1666998 173WT
GSM1666999 174WT
GSM1667000 194WT
This SubSeries is part of SuperSeries:
GSE63130 Next Generation Sequencing Analysis of Wild Type and Rfx2-/- Testicular Transcriptomes
SRA SRP057696
BioProject PRJNA282463

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Supplementary file Size Download File type/resource
GSE68283_Raw_counts.txt.gz 380.4 Kb (ftp)(http) TXT
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