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Series GSE68282 Query DataSets for GSE68282
Status Public on Nov 02, 2015
Title Next Generation Sequencing Analysis of Wild Type and Rfx2-/- Testicular Transcriptomes [ChIP-Seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Purpose: This study was carried out to determine the consequences of the Rfx2-/- genotype on spermatogenesis in the mouse
Methods: DNA was extracted from decapsulated testes of 21 day old mice. ChIP sequencing was used to determine the binding sites of RFX2.
Results: ChIP-Seq analysis identified ~880 binding sites of RFX2 near TSS.
Conclusion: Spermatogenesis undergoes complete arrest just prior to the end of the round spermatid period of sperm development in mutant mice. Sequencing results showed that approximately 105 genes were downregulated 2 fold or more in the testes of mutant mice. Comparison of similar studies of targeted mutations in genes for other transcription factor demonstrate that Rfx2 has a large and nearly unique set of genes that depend on it directly or indirectly. A large number of downregulated genes are identified with cilia function.
Overall design Testicular RFX2 binding sites were determined by deep sequencing using testes from 2 independentWT mice
Contributor(s) Kistler WS, Baas D, Lemeille S, Paschaki M, Barras E, Ma W, Duteyrat J, Morlé L, Durand B, Reith W
Citation(s) 26162102
Submission date Apr 27, 2015
Last update date May 15, 2019
Contact name Sylvain LEMEILLE
Organization name University of Geneva Medical School
Department Department of Pathology and Immunology
Street address 1 rue Michel-Servet
City Geneva
ZIP/Postal code 1211
Country Switzerland
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (4)
GSM1666994 input_WT_exp1
GSM1666995 input_WT_exp2
GSM1666996 RFX2_WT_exp1
This SubSeries is part of SuperSeries:
GSE63130 Next Generation Sequencing Analysis of Wild Type and Rfx2-/- Testicular Transcriptomes
SRA SRP057695
BioProject PRJNA282464

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Supplementary file Size Download File type/resource
GSE68282_RAW.tar 375.4 Mb (http)(custom) TAR (of BED, BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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