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Series GSE68255 Query DataSets for GSE68255
Status Public on Apr 06, 2016
Title Innate Immune Recognition of Bacterial Viability Instructs Human T follicular Helper Cell Differentiation
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Live attenuated vaccines are often superior to dead vaccines, yet the immunological mechanisms remain largely obscure. We have recently uncovered an inherent capacity of antigen-presenting cells (APC) to discriminate live from killed bacteria by virtue of vita-PAMPs. Here we found that innate recognition of bacterial viability strongly promotes the differentiation of fully functional T follicular helper (TFH) cells. We identify TLR8 and its signaling adaptor MyD88 as critical sensor for bacterial viability in human APC, activation of which is required and sufficient to induce selective transcriptional remodeling and the production of TFH promoting signals like IL-12. Activators of other TLRs including licensed vaccine adjuvants fail to do so. Consequently, vita-PAMP receptors such as TLR8 represent promising targets for adjuvants to improve the efficacy of modern inanimate subunit vaccines.
Overall design Human monocytes were infected with live or heat-killed E. coli for 6h.
Contributor(s) Valai A, Ugolini M, Gerhard J, Georg P, Helbig ET, Opitz B, Kurth F, Boekschoten MV, Müller M, Suttorp N, Sander LE
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Submission date Apr 24, 2015
Last update date Nov 08, 2016
Contact name Guido Hooiveld
Organization name Wageningen University
Department Div. Human Nutrition & Health
Lab Nutrition, Metabolism & Genomics Group
Street address HELIX, Stippeneng 4
City Wageningen
ZIP/Postal code NL-6708WE
Country Netherlands
Platforms (1)
GPL11532 [HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version]
Samples (12)
GSM1666321 Monocytes, 6hr after control incubation, subject 21
GSM1666322 Monocytes, 6hr after incubation with heat-killed E. coli, subject 27
GSM1666323 Monocytes, 6hr after incubation with live E. coli, subject 21
BioProject PRJNA282140

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Supplementary file Size Download File type/resource
GSE68255_RAW.tar 50.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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