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| Status |
Public on Apr 06, 2016 |
| Title |
Innate Immune Recognition of Bacterial Viability Instructs Human T follicular Helper Cell Differentiation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Live attenuated vaccines are often superior to dead vaccines, yet the immunological mechanisms remain largely obscure. We have recently uncovered an inherent capacity of antigen-presenting cells (APC) to discriminate live from killed bacteria by virtue of vita-PAMPs. Here we found that innate recognition of bacterial viability strongly promotes the differentiation of fully functional T follicular helper (TFH) cells. We identify TLR8 and its signaling adaptor MyD88 as critical sensor for bacterial viability in human APC, activation of which is required and sufficient to induce selective transcriptional remodeling and the production of TFH promoting signals like IL-12. Activators of other TLRs including licensed vaccine adjuvants fail to do so. Consequently, vita-PAMP receptors such as TLR8 represent promising targets for adjuvants to improve the efficacy of modern inanimate subunit vaccines.
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| Overall design |
Human monocytes were infected with live or heat-killed E. coli for 6h.
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| Contributor(s) |
Valai A, Ugolini M, Gerhard J, Georg P, Helbig ET, Opitz B, Kurth F, Boekschoten MV, Müller M, Suttorp N, Sander LE |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Apr 24, 2015 |
| Last update date |
Nov 08, 2016 |
| Contact name |
Guido Hooiveld |
| E-mail(s) |
guido.hooiveld@wur.nl
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| Organization name |
Wageningen University
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| Department |
Div. Human Nutrition & Health
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| Lab |
Nutrition, Metabolism & Genomics Group
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| Street address |
HELIX, Stippeneng 4
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| City |
Wageningen |
| ZIP/Postal code |
NL-6708WE |
| Country |
Netherlands |
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| Platforms (1) |
| GPL11532 |
[HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version] |
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| Samples (12)
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| GSM1666321 |
Monocytes, 6hr after control incubation, subject 21 |
| GSM1666322 |
Monocytes, 6hr after incubation with heat-killed E. coli, subject 27 |
| GSM1666323 |
Monocytes, 6hr after incubation with live E. coli, subject 21 |
| GSM1666324 |
Monocytes, 6hr after incubation with heat-killed E. coli, subject 21 |
| GSM1666325 |
Monocytes, 6hr after control incubation, subject 23 |
| GSM1666326 |
Monocytes, 6hr after incubation with live E. coli, subject 23 |
| GSM1666327 |
Monocytes, 6hr after control incubation, subject 35 |
| GSM1666328 |
Monocytes, 6hr after incubation with heat-killed E. coli, subject 23 |
| GSM1666329 |
Monocytes, 6hr after incubation with live E. coli, subject 35 |
| GSM1666330 |
Monocytes, 6hr after control incubation, subject 27 |
| GSM1666331 |
Monocytes, 6hr after incubation with heat-killed E. coli, subject 35 |
| GSM1666332 |
Monocytes, 6hr after incubation with live E. coli, subject 27 |
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| Relations |
| BioProject |
PRJNA282140 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE68255_RAW.tar |
50.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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