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Series GSE68177 Query DataSets for GSE68177
Status Public on Sep 02, 2015
Title Cell type transcriptomics of hypothalamic energy-sensing neuron responses to fasting
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary AGRP neurons are a hypothalamic population that senses physiological energy deficit and consequently increases appetite. Molecular and cellular processes for energy-sensing and elevated neuronal output are critical for understanding the central nervous system response to energy deficit states, such as during weight-loss. Cell type-specific transcriptomics can be used to identify pathways that counteract weight-loss but, in adult mice, this has been limited by technical challenges. We report high-quality gene expression profiles of AGRP neurons under well-fed and energy deficit states. For comparison, we also analyzed POMC neurons, an intermingled population that suppresses appetite. This data newly identifies cell type-selective involvement of signaling pathways, ion channels, neuropeptides, and G-protein coupled receptors. Combined with methods to validate and manipulate these pathways, this resource greatly expands molecular insight into neuronal regulation of body weight, and may be useful for devising therapeutic strategies for obesity and eating disorders.
Overall design Examination of 2 different neuronal cell types under 2 conditions.
Contributor(s) Sugino K, Henry T, Sternson S
Citation(s) 26329458
Submission date Apr 22, 2015
Last update date May 15, 2019
Contact name Ken Sugino
Organization name Janelia Research Campus
Lab NeuroSeq
Street address 19700 Helix Dr
City Ashburn
State/province VA
ZIP/Postal code 20147
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (21)
GSM1665110 Agrp fed rep1
GSM1665111 Agrp fed rep2
GSM1665112 Agrp fed rep3
BioProject PRJNA281954
SRA SRP057574

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE68177_Agrp.Pomc.fedfast.counts.txt.gz 1.6 Mb (ftp)(http) TXT
GSE68177_Agrp.Pomc.fedfast.tpm.txt.gz 2.0 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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