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Series GSE6776 Query DataSets for GSE6776
Status Public on Feb 23, 2007
Title Halobacterium NRC-1 Growth curve and GTF perturbation
Organism Halobacterium salinarum NRC-1
Experiment type Expression profiling by array
Summary Total RNA was collected at 6 or 8 points throughout the growth curve of Halobacterium NRC1 for wild-type, non-native expression of general transcription factors and knock-out strains of general transcription factors. This RNA was hybridized against our standard reference RNA (wild-type OD600 0.5-0.6).
Keywords: growth genetic modification
 
Overall design Total RNA was collected at 3 or 4 points throughout the growth-curve for each strain. Biological duplicates were grown and collected from for each strain making a total of 6 or 8 observations throughout the growth curve.

A total of 161 biological samples make up this experiment.

Strains include (wild-type NRC-1, delta-ura3 knockout control strain, pMTFcmyc plasmid-only control strain, TFBa,b,c,d and e knockout strains, TFBb, c, and d knock-out strains, cmycTFBa, b, c, d, e, f and g non-native expression strains, and cmycTBPa, b, c, d, e, f non-native expression strains).

Samples were hybridized against in-house spotted oligo arrays. Each sample is represented on two arrays as dye swaps.
 
Contributor(s) Facciotti MT, Pan M, Kaur A, Vuthoori M, Reiss DJ, Bonneau R, Shannon P, Srivastava A, Donahoe S, Hood L, Baliga NS
Citation(s) 17360575
Submission date Jan 17, 2007
Last update date Jan 15, 2017
Contact name Marc Facciotti
E-mail(s) mfacciotti@systemsbiology.org
Organization name Institute for Systems Biology
Street address 1441 North 34th Street
City Seattle
State/province WA
ZIP/Postal code 98103
Country USA
 
Platforms (1)
GPL3739 ISB Halobacterium Spotted Microarray
Samples (322)
GSM156188 Halobacterium NRC-1 with pMTFcmyc expressing Cmyc grown to OD600 0.175 with mevinolin, Array 9986
GSM156189 Halobacterium NRC-1 with pMTFcmyc expressing Cmyc grown to OD600 0.175 with mevinolin, Array 9987
GSM156190 Halobacterium NRC-1 with pMTFcmyc expressing Cmyc grown to OD600 0.179 with mevinolin, Array 9988
Relations
BioProject PRJNA98919

Download family Format
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Supplementary data files not provided

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