|
| Status |
Public on Apr 15, 2015 |
| Title |
Myc and SAGA Rewire an Alternative Splicing Network During Early Somatic Cell Reprogramming [Reprogramming_RNASEQ] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Embryonic stem cells are maintained in a self-renewing and pluripotent state by multiple regulatory pathways. Pluripotent-specific transcriptional networks are sequentially reactivated as somatic cells reprogram to achieve pluripotency. How epigenetic regulators modulate this process and contribute to somatic cell reprogramming is not clear. Here we perform a functional RNAi screen to identify the earliest epigenetic regulators required for reprogramming. We identify components of the SAGA histone acetyltransferase complex, in particular Gcn5, as critical regulators of reprogramming initiation. Furthermore, we show in mouse pluripotent stem cells that Gcn5 strongly associates with Myc and that upon initiation of somatic reprogramming, Gcn5 and Myc form a positive feed forward loop that activates a distinct alternative splicing network and the early acquisition of pluripotency-associated splicing events. These studies expose a Myc-SAGA pathway that drives expression of an essential alternative splicing regulatory network during somatic cell reprogramming.
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| |
|
| Overall design |
Examination of expression level changes at D0 and D2 MEFs
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| |
|
| Contributor(s) |
Hirsch C, Coban Akdemir ZH |
| Citation(s) |
25877919 |
| |
| Submission date |
Apr 09, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Sharon Y.R. Dent |
| Organization name |
The University of Texas MD Anderson Cancer Center
|
| Department |
Dept. of Molecular Carcinogenesis
|
| Street address |
1800 Park Road 1C Unit Number: 0116 Smithville, TX 78957 Room Number: SRC1.104
|
| City |
Smithville |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (12)
|
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| This SubSeries is part of SuperSeries: |
| GSE67719 |
Myc and SAGA Rewire an Alternative Splicing Network During Early Somatic Cell Reprogramming |
|
| Relations |
| BioProject |
PRJNA280754 |
| SRA |
SRP057021 |
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