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| Status |
Public on Jan 22, 2007 |
| Title |
1,25 (OH)2 vitamin D3 induces expression of CCR10 and other genes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Human naive T cells from peripheral blood were cultured in 24 wells coated with anti-CD3 and anti-CD28 antibodies in the presence or absence of retinoid acid, IL-12, and 1,25 (OH)2 vitamin D3. The T cells were FACS-sorted based on expression of CD3, integrin alpha4beta7, cutaneous lymphocyte antigen (CLA) and chemokine receptor 10. This serie includes microarray data from stimulated T cells under indicated conditions.
Keywords: Human T cell, vitamin D and A, chemokine receptor, nuclear receptor
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| Overall design |
Total RNA was isolated from these sorted T cells using Invitrogen RNeasy mini-kit. The quality of RNA was prechecked by Agilent BioAnalyzer. Double-stranded cDNA was synthesized, and transcribed into Biotin-labeled cRNA, which was fragmented before hybridization. All the procedure was performed in the Stanford University PAN facility.
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| Contributor(s) |
Pan J |
| Citation(s) |
17259988 |
| Submission date |
Jan 13, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Junliang Pan |
| E-mail(s) |
jpan@stanford.edu
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| Phone |
510-705-4011
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| Organization name |
Stanford University
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| Department |
Pathology
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| Street address |
3801 Miranda Avenue
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| City |
Palo Alto |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (6)
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| GSM153893 |
Retinoid Acid stimulated T effector cells |
| GSM154073 |
Unstimulated T effector cells |
| GSM154077 |
IL-12 stimulated T effector cells |
| GSM154078 |
1,25(OH)2 vitamin D3 stimulated T effector cells |
| GSM154081 |
1,25(OH)2 vitamin D3 plus IL-12 stimulated CCR10-positive CLA-positive T cells |
| GSM154084 |
1,25(OH)2 vitamin D3 plus IL-12 stimulated CCR10-negative CLA-positive T cells |
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| Relations |
| BioProject |
PRJNA98981 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE6743_RAW.tar |
29.5 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data provided as supplementary file |
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