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| Status |
Public on Dec 31, 2015 |
| Title |
Comparing two intestinal porcine epithelial cell lines (IPECs): global expression patterns to characterise a in vitro model of intestinal physiology |
| Organism |
Sus scrofa |
| Experiment type |
Expression profiling by array
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| Summary |
The intestinal epithelial cell lines 1 and J2 (IPEC-1, IPEC-J2) - spontaneously immortalised cell lines from the porcine intestine - are important tools for studying intestinal function. Microarrays (GeneChip Porcine Genome Array) were used to compare the expression pattern at basal in vitro conditions. Expression analyses complemented by morphological, functional and biochemical analyses revealed that IPEC-J2 is a morphologically and functionally more differentiated cell line in comparison to IPEC-1. In addition, IPEC-2 cells are a preferential tool for in vitro studies with the focus on metabolism.
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| Overall design |
We compared the genome wide gene expression of untreated and IPEC 1 an dIPEC-J2 cells by the GeneChip® Porcine Genome Array of Affymetrix.
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| Contributor(s) |
Nossol C, Barta-Böszörményi A, Kahlert S, Zuschratter W, Faber-Zuschratter H, Reinhardt N, Ponsuksili S, Wimmers K, Diesing A, Rothkötter H |
| Citation(s) |
26147118 |
| |
| Submission date |
Mar 30, 2015 |
| Last update date |
Apr 29, 2016 |
| Contact name |
Klaus Wimmers |
| E-mail(s) |
wimmers@fbn-dummerstorf.de
|
| Phone |
03820868600
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| Organization name |
FBN Dummerstorf
|
| Department |
Genome Biology
|
| Street address |
Wilhelm-Stahl-Allee 2
|
| City |
Dummerstorf |
| ZIP/Postal code |
18196 |
| Country |
Germany |
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| Platforms (1) |
| GPL3533 |
[Porcine] Affymetrix Porcine Genome Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA279842 |
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