|
| Status |
Public on May 29, 2015 |
| Title |
Dnmt1 is essential to maintain progenitors in the perinatal intestinal epithelium. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report that Dnmt1 is crucial during perinatal intestinal development. Loss of Dnmt1 in intervillus progenitor cells causes global hypomethylation, DNA damage, premature differentiation, and apoptosis, and consequently, loss of nascent villi. We further confirm the critical role for Dnmt1 during crypt development using the in vitro organoid culture system, and illustrate a clear differential requirement for Dnmt1 in immature versus mature organoids. These results demonstrate an essential role for Dnmt1 in maintaining genomic stability during intestinal development and the establishment of intestinal crypts.
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| |
|
| Overall design |
We performed RNA-Seq of control and Dnmt1-ablated intestinal progenitor cells isolated from parrafin embedded tissues by laser capture microdissection (LCM).
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| |
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| Contributor(s) |
Elliott EN, Kaestner KH |
| Citation(s) |
26023099 |
| |
| Submission date |
Mar 27, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Ellen Elliott |
| E-mail(s) |
wellen@mail.med.upenn.edu
|
| Phone |
215-898-8712
|
| Organization name |
University of Pennsylvania Perelman School of Medicine
|
| Department |
Genetics
|
| Lab |
Klaus Kaestner Lab
|
| Street address |
12-164 Smilow TRC, 3400 Civic Center Blvd
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (5)
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|
| Relations |
| BioProject |
PRJNA279588 |
| SRA |
SRP056630 |
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