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| Status |
Public on Apr 13, 2015 |
| Title |
Functional Antagonism Between CELF and Mbnl Proteins in Cytoplasm and Nucleus [RNA Bind-N-Seq] |
| Organism |
synthetic construct |
| Experiment type |
Other
|
| Summary |
The CUG-BP and ETR-3-like factor 1 (Celf1) RNA binding protein plays an important role in heart and muscle development, and is over-expressed in the disease myotonic dystrophy. Celf1 has known roles in regulation of RNA splicing, RNA stability, and protein translation. To identify transcriptome-wide targets of the Celf1 protein, we performed CLIP-seq against Celf1 using the 3B1 antibody, in myoblasts, heart tissue, and muscle tissue.
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| Overall design |
RNA Bind-N-Seq was performed using recombinant CELF1 protein in the presence of competing amounts of recombinant MBNL1 protein.
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| Contributor(s) |
Wang ET, Ward A, Cherone J, Giudice J, Wang TT, Treacy D, Freese P, Lambert N, Saxena T, Cooper T, Burge C |
| Citation(s) |
25883322 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| DP5 OD017865 |
Post-transcriptional regulation of gene expression in neuromuscular disease: Ppst-transcriptional regulation of gene expression in neuromuscular disease |
MASSACHUSETTS INSTITUTE OF TECHNOLOGY |
ERIC T WANG |
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| |
| Submission date |
Mar 26, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Eric T Wang |
| Organization name |
University of Florida
|
| Street address |
2033 Mowry Rd
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL15228 |
Illumina HiSeq 2000 (synthetic construct) |
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| Samples (7)
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| This SubSeries is part of SuperSeries: |
| GSE61893 |
Functional Antagonism Between CELF and Mbnl Proteins in Cytoplasm and Nucleus |
|
| Relations |
| BioProject |
PRJNA279533 |
| SRA |
SRP056608 |
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