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| Status |
Public on Jun 30, 2019 |
| Title |
Identifying signals from the skeletal muscle niche that regulate satellite cell function |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Skeletal muscle is the most common tissue in the body. Its continued maintenance and regeneration throughout life is essential to the function of the organism. Satellite cells are critical to regeneration of the skeletal muscle and strategies to improve function of satellite cells are of great importance. Muscle-resident Fibro-Adipogenic Precursors (FAPs) cells are a critical component of the satellite cell niche and help orchestrate efficient muscle regeneration and potentiate satellite cell differentiation via soluble or secreted factors. Populations with similar phenotype and function to muscle FAPs have been isolated from the skin and white adipose tissue. Interactions between tissue-specific FAP cells and resident stem cells in those tissues might be conserved. Therefore, defining specific factors that mediate the relationship between muscle FAPs and satellite cells would have implications for other organs.
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| Overall design |
Use parabiosis as a model to determine if muscle FAPs can enter cross-circulation. Perform RNA sequencing of muscle and lung FAPs to identify transcripts that stimulate satellite cell differentiation
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| Contributor(s) |
Wagers A, Manohar R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 20, 2015 |
| Last update date |
Jun 30, 2019 |
| Contact name |
Paul A Wilson |
| E-mail(s) |
paul.a.wilson@gsk.com
|
| Organization name |
GSK
|
| Department |
Target Sciences
|
| Lab |
Computational Biology
|
| Street address |
GlaxoSmithKline Medicine Research Centre
|
| City |
Stevenage |
| State/province |
Hertfordshire |
| ZIP/Postal code |
SG1 2NY |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (13)
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| Relations |
| BioProject |
PRJNA278988 |
| SRA |
SRP056384 |
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