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| Status |
Public on Sep 15, 2015 |
| Title |
A novel phosphate-starvation response in fission yeast requires the endocytic function of Myosin I (ChIP-seq) |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
S. pombe Myosin I (MyoI) plays a key role in cytoskeletal functions, in particular it is part of the endocytotic machinery. Here we show a novel role for MyoI in regulating the response to low phosphate stress. Deletion of MyoI led to an increased proliferation in low phosphate media when compared to a control strain; mutations in other proteins involved in the early steps of endocytosis, such as ARP2 and Sla2/END4, led to the same phenotype. We show that once cells are deprived of phosphate they undergo clear morphological changes, slow down growth but retain viability. This is reminiscent of a quiescence response, and it is completely abolished when MyoI is absent. Transcriptomic analysis revealed a wide perturbation of gene expression upon phosphate starvation, with rapid induction of stress-regulated genes. However, Æmyo1 cells displayed little or negligible effects at gene expression level when they were phosphate-starved. Using a combination of super resolution microscopy and genetic approaches we pinpointed the specific function of MyoI in endocytosis as critical for the response to low phosphate stress. Furthermore, introduction of point-mutated MyoI (with ablated endocytic function) into a ÆmyoI background failed to restore phosphate stress response. Taken together, our data indicate how endocytosis, and in particular MyoI, play a pivotal role in mediating cellular response to nutrients, bridging the external environment and internal molecular functions of the cells.
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| |
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| Overall design |
ChIP-seq analysis of Myo1-GFP genomic binding in control or low phosphate condition
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| |
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| Contributor(s) |
Petrini E, Baillet V, Cridge J, Hogan C, Guillaume C, Ke H, Brandetti E, Koohy H, Spivakov M, Varga-Weisz P |
| Citation(s) |
26345368 |
| |
| Submission date |
Mar 20, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Felix Krueger |
| E-mail(s) |
fkrueger@altoslabs.com
|
| Organization name |
Altos Labs
|
| Department |
Bioinformatics
|
| Street address |
Granta Park
|
| City |
Cambridge |
| ZIP/Postal code |
CB21 6GP |
| Country |
United Kingdom |
| |
|
| Platforms (2) |
| GPL16192 |
Illumina MiSeq (Schizosaccharomyces pombe) |
| GPL17225 |
Illumina HiSeq 2500 (Schizosaccharomyces pombe) |
|
| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE67126 |
A new phosphate-starvation response in fission yeast requires the endocytic function of myosin I |
|
| Relations |
| BioProject |
PRJNA278998 |
| SRA |
SRP056389 |
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