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Series GSE6708 Query DataSets for GSE6708
Status Public on May 01, 2008
Title Nucleus- and cell-specific gene expression in monkey thalamus
Platform organism Homo sapiens
Sample organism Macaca mulatta
Experiment type Expression profiling by array
Summary Nuclei of the mammalian thalamus are aggregations of neurons with unique architectures and input-output connections, yet the molecular determinants of their organizational specificity remain unknown. By comparing expression profiles of thalamus and cerebral cortex in adult rhesus monkeys we identified transcripts that are unique to dorsal thalamus or to individual nuclei within it. Real-time quantitative polymerase chain reaction and in situ hybridization analyses confirmed the findings. Expression profiling of individual nuclei microdissected from the dorsal thalamus revealed additional subsets of nucleus-specific genes. Functional annotation using Gene Ontology (GO) vocabulary and Ingenuity Pathway analysis revealed over-representation of GO categories related to development, morphogenesis, cell-cell interactions, and extracellular matrix within the thalamus- and nucleus-specific genes-many involved in the Wnt signaling pathway. Examples included the transcription factor TCF7L2, localized exclusively to excitatory neurons, a calmodulin-binding protein PCP4, the bone extracellular matrix molecules SPP1 and SPARC, and other genes involved in axon outgrowth and cell matrix interactions. Other nucleus-specific genes such as CBLN1 are involved in synaptogenesis. The genes identified likely underlie nuclear specification, cell phenotype and connectivity during development and their maintenance in the adult thalamus.
Keywords: brain region comparative analysis
Overall design To determine the molecular underpinnings of nuclear specificity in the dorsal thalamus we isolated micro-punches of tissue from nucleus-specific regions and processed them for microarray analysis. Replicate samples from 5 separate dorsal thalamic nuclei were processed and compared to identify genes unique to each region. Affymetrix U133A Gene Chips were used. All of the samples were isolated from untreated adult monkey brain.
Contributor(s) Murray KD, Choudary PV, Jones EG
Citation(s) 17261798
Submission date Jan 10, 2007
Last update date Aug 10, 2018
Contact name Karl Daniel Murray
Organization name UC Davis
Street address 1544 Newton Court
City Davis
State/province CA
ZIP/Postal code 95618
Country USA
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (10)
GSM154729 Anterior Thalamus Replicate 1
GSM154731 Anterior Thalamus Replicate 2
GSM154732 Centre Median Replicate 1
BioProject PRJNA99027

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