 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on May 15, 2015 |
| Title |
Brg1 activates enhancer repertoires to establish B cell identity and modulate cell growth |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
|
| Summary |
Early B cell development is orchestrated by the combined activities of the transcriptional regulators E2A, EBF1, Foxo1 and Ikaros. However, how the genome-wide binding patterns of these regulators are modulated during B-lineage development remains to be determined. Here, we found that in lymphoid progenitors the chromatin remodeler Brg1 specified the B cell fate. In committed pro-B cells Brg1 regulated Igh locus contraction and controlled c-Myc expression to modulate the expression of genes that regulate ribosome biogenesis. In committed pro-B cells Brg1 also suppressed a pre-B lineage-specific pattern of gene expression. Finally, we found that Brg1 acted mechanistically to establish B cell fate and modulate cell growth by facilitating access of lineage-specific transcription factors to poised enhancer repertoires.
|
| |
|
| Overall design |
8 ATAC-Seq samples from sorted ALP and BLP (duplicates, control and Brg1-deleted), 4 ATAC-Seq samples from cultured pro-B cells (duplicates, control and Brg1-deleted), 2 Ikaros ChIP-seq samples (performed in Rag1-/- pro-B cells and in E2A-/- pre-pro-B cells), 1 Brg1 ChIP-seq sample and accompanying Input sample (both in Rag1-/- pro-B cells), 4 RNA-Seq samples from cultured pro-B cells (duplicates, control and Brg1-deleted), 6 RNA-Seq samples from cultured Rag1-/- pro-B cells (triplicates, control and Brg1-knock down).
|
| |
|
| Contributor(s) |
Bossen C, Murre CS, Chang AN, Mansson R, Rodewald H, Murre C |
| Citation(s) |
25985234 |
| |
| Submission date |
Mar 17, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Claudia Bossen |
| E-mail(s) |
claudia.bossen@gmail.com
|
| Organization name |
University Hospital Freiburg (D)
|
| Department |
Center for Chronic Immunodeficiency
|
| Lab |
Claudia Bossen
|
| Street address |
Breisacher Strasse 115
|
| City |
Freiburg |
| ZIP/Postal code |
79106 |
| Country |
Germany |
| |
|
| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (26)
|
|
| Relations |
| BioProject |
PRJNA278629 |
| SRA |
SRP056238 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE66978_ALP-ctrl-all.ucsc.bedGraph.gz |
78.3 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE66978_ALP-ko-all.ucsc.bedGraph.gz |
82.2 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE66978_BLP-ctrl-all.ucsc.bedGraph.gz |
77.2 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE66978_BLP-ko-all.ucsc.bedGraph.gz |
81.6 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE66978_ProB-ctrl-all.ucsc.bedGraph.gz |
81.3 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE66978_ProB-ko-all.ucsc.bedGraph.gz |
78.7 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE66978_ProB_Brg1ko_vs_ProB_ctrl.txt.gz |
1.0 Mb |
(ftp)(http) |
TXT |
| GSE66978_RAW.tar |
209.3 Mb |
(http)(custom) |
TAR (of BEDGRAPH) |
| GSE66978_Rag1ko_Brg1kd_vs_Rag1ko_ctrl.txt.gz |
1.0 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...