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Series GSE66939 Query DataSets for GSE66939
Status Public on Oct 18, 2015
Title Paf1 complex factors, Leo1 and Paf1, promote local histone turnover to modulate chromatin states in fission yeast [ChIP-exo]
Organism Schizosaccharomyces pombe
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Maintenance of open and repressed chromatin states is crucial for regulation of gene expression. To study the genes involved in maintaining chromatin states we generated a random mutant library using the Hermes transposon mutagenesis system in fission yeast Schizosacchromyces pombe. The silencing of reporter genes inserted in the euchromatic region adjacent to the heterochromatic mating type locus was monitored. We identified Leo1-Paf1, a subcomplex of the RNA Polymerase II Associated Factor 1 Complex (Paf1C), required to prevent spreading of heterochromatin into euchromatin. Through high-resolution genome-wide ChIP (ChIP-exo) we mapped the heterochromatin mark H3K9me2 in leo1∆ cells. Loss of Leo1-Paf1 led to increased heterochromatin stability at several facultative heterochromatin loci. The RNAi machinery is the major pathway for heterochromatin formation in S. pombe. However, small RNA sequencing showed that heterochromatin assembly in leo1∆ cells was RNAi-independent. By examining histone turnover rate in leo1∆ cells, we showed that deletion of Leo1 decreased nucleosome turnover, which led to heterochromatin spreading. Our data revealed that Leo1-Paf1 promotes chromatin state fluctuations by enhancing histone turnover.
Overall design H3K9me2 ChIP experiments performed on wild-type and leo1::kan strains. Two-replicates each.
Contributor(s) Sadeghi L, Prasad P, Ekwall K, Cohen A, Svensson JP
Citation(s) 26518661
Submission date Mar 16, 2015
Last update date May 15, 2019
Contact name Karl Ekwall
Phone +46 8 6089133
Organization name Karolinska Inst
Street address Alfred Nobels Alle 7
City Stockholm
ZIP/Postal code S-141 89
Country Sweden
Platforms (1)
GPL13988 Illumina HiSeq 2000 (Schizosaccharomyces pombe)
Samples (4)
GSM1634684 leo1_H3K9me2 rep1
GSM1634685 leo1_H3K9me2 rep2
GSM1634686 wt_H3K9me2 rep1
This SubSeries is part of SuperSeries:
GSE66941 Paf1 complex factors, Leo1 and Paf1, promote local histone turnover to modulate chromatin states in fission yeast
BioProject PRJNA278409
SRA SRP056209

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Supplementary file Size Download File type/resource
GSE66939_leo1_H3K9me2_res25.wig.gz 116.3 Kb (ftp)(http) WIG
GSE66939_wt_H3K9me2_res25.wig.gz 32.9 Kb (ftp)(http) WIG
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