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Series GSE66798 Query DataSets for GSE66798
Status Public on Mar 01, 2016
Title The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary It is well known that both recipient cells and donor nuclei demonstrate a mitotic advantage as observed in the traditional reprogramming with somatic cell nuclear transfer (SCNT). However, It is not known whether a specific mitotic factor plays a critical role in reprogramming. Here we identify an isoform of human bromodomain-containing 3 (BRD3), BRD3R (BRD3 with Reprogramming activity), as a reprogramming factor. BRD3R positively regulates mitosis during reprogramming, upregulates a large set of mitotic genes at early stages of reprogramming, and associates with mitotic chromatin. Interestingly, a set of the mitotic genes upregulated by BRD3R constitutes a pluripotent molecular signature. The two BRD3 isoforms display differential binding to acetylated histones. Our results suggest a molecular interpretation for the mitotic advantage in reprogramming, and show that mitosis may be a driving force of reprogramming.
Overall design Human BJ cells transduced with lentiviral particles of the conventional reprogramming factors (OCT3/4, SOX2 and KLF4) were used as controls. Two types of controls were used: 1) BJ transduced with OSK (OCT4, SOX2 and KFL4) viruses; 2) BJ cells transduced with OSK plus GFP viruses. Experimental treatment was BJ cells transduced with OSK plus BRD3R viruses. RNA was extracted from cells at day 3 of reprogramming because the reprogramming cells are still homogeneous and transgenes are well expressed at this time point.
Contributor(s) Khodadadi-Jamayran A, Shao Z, Crowley M, Crossman D, Hu K
Citation(s) 26947130
Submission date Mar 11, 2015
Last update date Apr 06, 2020
Contact name Alireza Khodadadi-Jamayran
Organization name New York University, NYU Langone Medical Center
Department Division of Advanced Research Technologies (DART)
Lab Applied Bioinformatics Laboratories (ABL)
Street address 550 1st Ave, MSB 304
City New York City
State/province NY
ZIP/Postal code 10016
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (14)
GSM1632422 BJ-OSK-A
GSM1632423 BJ-OSK-B
BioProject PRJNA277980
SRA SRP056087

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Supplementary file Size Download File type/resource
GSE66798_Normolized6samples.xls.gz 1.1 Mb (ftp)(http) XLS
GSE66798_Normolized8samples.xls.gz 1.4 Mb (ftp)(http) XLS
GSE66798_RAW.tar 1.9 Gb (http)(custom) TAR (of BW)
GSE66798_merged_counts6samples.xls.gz 532.1 Kb (ftp)(http) XLS
GSE66798_merged_counts8samples.xls.gz 635.6 Kb (ftp)(http) XLS
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