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| Status |
Public on Feb 19, 2016 |
| Title |
Development of coregulator-dependent androgen receptor target gene signatures |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To decipher the contribution of coregulators to androgen receptor target gene expression, effect of siRNA-mediated silencing of 18 clinically relevant coregulators on bona fide androgen receptor target gene expression was studied.
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| Overall design |
Human LNCaP prostate cancer cells were transfected with individual siRNA SmartPools targeting 18 clinically relevant coregulators or a non-targeting siRNA SmartPool. Forty-two hours after transfection, cells were treated with synthetic androgen R1881 (5nM) or vehicle. Three biological replicates were generated per treatment group. Forty-eight hours later, total RNA was isolated and processed for Agilent microarray analysis.
The two normalizer are aliquots from same RNA sample that serves as internal control.
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| Contributor(s) |
Heemers H, Wang D |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 10, 2015 |
| Last update date |
Feb 20, 2016 |
| Contact name |
Dan Wang |
| E-mail(s) |
wangdan412@gmail.com
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| Organization name |
RPCI
|
| Street address |
Elm & Carlton Streets
|
| City |
buffalo |
| State/province |
NY |
| ZIP/Postal code |
14263 |
| Country |
USA |
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| Platforms (1) |
| GPL19874 |
Agilent-029218 Heemers AR axis PCF 2010 |
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| Samples (140)
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| Relations |
| BioProject |
PRJNA277808 |
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