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Series GSE66612 Query DataSets for GSE66612
Status Public on Sep 30, 2015
Title Longitudinal Studies of a B-Cell-Derived Signature of Tolerance in Renal Transplantation
Organism Homo sapiens
Experiment type Expression profiling by RT-PCR
Summary The development of reliable biomarkers of transplant tolerance would enhance the safety and feasibility of clinical trials and potentially also facilitate management of patients on standard immunosuppressive therapies. To this end, we examined peripheral blood samples of spontaneously tolerant renal transplant recipients, as well as patients enrolled in two interventional tolerance trials, using multiparameter flow cytometry and gene expression analysis. Repeat analysis of samples from a previously reported tolerant cohort, as well as newly identified tolerant patients, confirmed that tolerance was associated with increased expression of B cell-associated genes relative to immunosuppressed patients with stable renal function. This was not accounted for merely by an increase in total B cell numbers, but appeared to be the result of increased frequencies of transitional and naïve B cells. Moreover, serial measurements demonstrated that this pattern persisted over several years. Importantly, patients rendered tolerant via induction of transient mixed chimerism, and those weaned to low levels of immunosuppression, showed similar increases in the expression of selected B cell genes as did spontaneously tolerant individuals. Collectively, these findings support the notion that alterations in B cells may be a common theme for tolerant kidney transplant recipients, and a useful immune monitoring tool in prospective trials.
The MassARRAY QGE (Sequenom) multiplexed primer and competitive template designs and analysis were reported previously.
 
Overall design Total of 25 TOL (Tolerant), 7 New TOL (New Tolerant), 34 SI (Standard Immunosuppression) in cohort ITN507; 7 Sirolimus Monotherapy, 2 SI Multiagent in cohort ITN013; and 7 TOL and 1 Return to SI in cohorts ITN010 and ITN036.
 
Contributor(s) Newell KA, Sanz I, Wei C, Rosenberg A, Gao Z, Kanaparthi S, Asare S, Lim N, Stahly M, Howell M, Knechtle S, Kirk A, Marks WH, Kawai T, Spitzer T, Tolkoff-Rubin N, Sykes M, Sachs DH, Cosimi AB, Burlingham WJ, Asare A, Phippard D, Turka LA
Citation(s) 26461968
Submission date Mar 06, 2015
Last update date Jan 05, 2016
Contact name Adam Asare
E-mail(s) aasare@immunetolerance.org
Organization name Immune Tolerance Network
Department Bioinformatics Group
Street address 7500 Old Georgetown Road, Suite 800
City Bethesda
State/province MD
ZIP/Postal code 20814
Country USA
 
Platforms (1)
GPL19858 MassARRAY QGE
Samples (158)
GSM1626147 SI, FACTOR_109622_002
GSM1626148 SI, FACTOR_109622_006
GSM1626149 TOL, FACTOR_118518_000
Relations
BioProject PRJNA277456

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE66612_non-normalized.txt.gz 281.6 Kb (ftp)(http) TXT
Processed data included within Sample table
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