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| Status |
Public on Jan 04, 2016 |
| Title |
Insights into responses of human astrocytes to H5N1 infection by transcriptional analysis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Increasing evidence demonstrates influenza virus can not only affect the respiratory system, but also infect CNS and lead to CNS disorder and encephalopathy and encephalitis. Astrocytes are the most abundant cells in the CNS, which are capable of producing cytokines and neurotrophic factors and are essential for brain homeostasis and neuronal function. Previous studies suggested that influenza virus can infect astrocytes and induce proinflammatory cytokines response as well as apoptosis. Nevertheless, very few mechanistic data are available regarding host responses to H5N1 infection in astrocytes. In this study, a functional genomics approach was utilized to investigate comprehensive host responses to H5N1 infection in a human astrocyte cell line, U251 cells.
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| Overall design |
U251 cells were infected by H5N1 at MOI 1 or control . At time points 6, 12, and 24h, total RNA were extracted for microarray experiment. Three replicates were performed at each time point of infection and control infection.
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| Contributor(s) |
Lin X, Wang R, Sun X, Zhang J, Jin M |
| Citation(s) |
26008703 |
| |
| Submission date |
Mar 06, 2015 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Lin xian |
| E-mail(s) |
yoya12@163.com
|
| Organization name |
Huazhong Agricutural University
|
| Department |
College of Veterinary Medicine
|
| Lab |
State Key Laboratory of Agricultural Microbiology
|
| Street address |
shizishan street,number 1
|
| City |
wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430070 |
| Country |
China |
| |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA277411 |
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